{"doi":"10.1101/2022.04.18.488657","title":"Localization, induction, and cellular effects of tau-phospho-threonine 217","abstract":"ABSTRACT Introduction Tau phosphorylation at T217 is a promising AD biomarker, but its functional consequences were unknown. Methods Human brain and cultured mouse neurons were analyzed by immunoblotting and immunofluorescence for total tau, tau pT217 , tau pT181 , tau pT231 and tau pS396/pS404 . dSTORM super resolution microscopy was used to localize tau pT217 in cultured neurons. EGFP-tau was expressed in fibroblasts as wild type and T217E pseudo-phosphorylated tau, and fluorescence recovery after photobleaching (FRAP) reported tau turnover rates on microtubules. Results In brain, tau pT217 appears in neurons at Braak stages I-II, becomes more prevalent later and co-localizes partially with other phospho-tau epitopes. In cultured neurons tau pT217 , is increased by extracellular tau oligomers (xcTauOs), and is associated with developing post-synaptic sites. FRAP recovery was fastest for EGFP-tau T217E . Conclusion Tau pT217 increases in brain as AD progresses and is induced by xcTauOs. Post-synaptic tau pT217 suggests a role for T217 phosphorylation in synapse impairment. T217 phosphorylation reduces tau’s affinity for microtubules.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":306982,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.96,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":891439,"name":"James W. Mandell","orcid":"0000-0002-5000-8276","position":1,"is_corresponding":false},{"id":411786,"name":"George S. Bloom","orcid":"0000-0003-4781-7627","position":2,"is_corresponding":false},{"id":1001559,"name":"Binita Rajbanshi","orcid":null,"position":0,"is_corresponding":true}],"reference_count":29,"raw_metadata":null,"created_at":"2026-07-19T00:32:52.703737Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}