{"doi":"10.1101/2022.04.16.488552","title":"Induction of a CD8 T cell intrinsic DNA damage and repair response is associated with clinical response to PD-1 blockade in uterine cancer","abstract":"Abstract Despite the success of immune checkpoint blockade (ICB), many patients fail to achieve durable clinical benefit, and the underlying immunological mechanisms remain poorly understood. Here, we investigated immune reinvigoration by ICB in advanced or recurrent hypermutated or microsatellite instability-high, mismatch repair deficient (MSI-H/MMRd) uterine cancer patients treated with anti-PD-1 (nivolumab). CD8 T cells underwent rapid pharmacodynamic proliferation 2-4 weeks after initiating PD-1 blockade. This immunological response, however, did not correlate with clinical response. We hypothesized that the T cell-intrinsic response to proliferative and genotoxic stress might contribute to the disparity between immunological and clinical response. We developed a high-dimensional single cell cytometric platform to simultaneously analyze T cell differentiation with changes in DNA damage and repair (DDR) pathways. This DDR-Immune platform revealed T cell subset-specific patterns of DDR, and distinct DDR pathways induced by different types of DNA damage. Applying this platform to MSI-H/MMRd or hypermutated uterine cancer patients revealed a signature of DDR exemplified by rapid increase in phosphorylated-ATM (pATM) intrinsic to CD8 T cells proliferating in response to PD-1 blockade that distinguished clinical responders and non-responders. ATM regulated transcriptional circuits in T cells were associated with better clinical response to PD-1 blockade. These findings highlight a previously unrecognized role for CD8 T cell-intrinsic DDR as a potential determinant of immune fitness and clinical outcome of PD-1 blockade therapy. STATEMENT OF SIGNIFICANCE Using high-dimensional immune and DNA damage response (DDR) profiling in T cell subsets we identified distinct signatures in anti-PD-1 treated MSI-H/hypermutated uterine cancer patients associated with clinical response versus non-response. Thus, T cell-intrinsic DDR is a potential determinant of immune responsiveness and clinical outcome to PD-1 blockade therapy in cancer.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":298627,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9626,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":105455,"name":"Sasikanth Manne","orcid":"0000-0001-9856-0871","position":1,"is_corresponding":false},{"id":617588,"name":"Nils Wellhausen","orcid":"0000-0001-8955-7582","position":2,"is_corresponding":false},{"id":105449,"name":"Derek A. Oldridge","orcid":"0000-0003-2177-5633","position":3,"is_corresponding":false},{"id":105450,"name":"Allison R. Greenplate","orcid":"0000-0002-2614-3072","position":4,"is_corresponding":false},{"id":855194,"name":"Lakshmi Chilukuri","orcid":null,"position":5,"is_corresponding":false},{"id":35343,"name":"Divij Mathew","orcid":"0000-0002-8323-7358","position":6,"is_corresponding":false},{"id":227831,"name":"Caiyue Xu","orcid":"0000-0003-4056-5586","position":7,"is_corresponding":false},{"id":72304,"name":"Ramin S. Herati","orcid":"0000-0003-2613-4050","position":8,"is_corresponding":false},{"id":227842,"name":"Shelley L. Berger","orcid":"0000-0001-5398-4400","position":9,"is_corresponding":false},{"id":105478,"name":"Alexander C. Huang","orcid":"0000-0002-0099-0492","position":10,"is_corresponding":false},{"id":227870,"name":"Carl H. June","orcid":"0000-0003-0241-3557","position":11,"is_corresponding":false},{"id":227533,"name":"Dmitriy Zamarin","orcid":"0000-0002-0094-0161","position":12,"is_corresponding":false},{"id":381100,"name":"Claire F. Friedman","orcid":"0000-0003-2621-8553","position":13,"is_corresponding":false},{"id":36451,"name":"E. John Wherry","orcid":"0000-0003-0477-1956","position":14,"is_corresponding":false},{"id":640100,"name":"Yuki Muroyama","orcid":"0000-0001-9839-6370","position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:31:36.269611Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}