{"doi":"10.1101/2022.04.06.487296","title":"Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Centrioles play critical roles in our cells, being part of centrosomes and cilia, which are important microtubule organising centers (MTOC) with a variety of roles. While centrioles are very stable structures, they disappear in certain cell types upon differentiation, such as in oocytes. Little is known about the regulation of centriole structural integrity. We previously uncovered that the pericentriolar material (PCM), and its recruiter Polo kinase, are required for both the maintenance of centriole structural integrity and centrosome MTOC activity. Using an hypothesis driven RNAi screen, we show that both the cartwheel and the centriole wall play an important role in centrosome integrity. In particular, we uncovered that the centriole wall protein ANA1 is critical for the integrity of both new and mature centrioles, in\n                  <jats:italic>Drosophila</jats:italic>\n                  oogenesis as well as in cultured cells. Moreover, our results show that the activity of both Polo and the PCM in centriole integrity depends on ANA1. Our work suggests that the structural integrity of centrioles, once thought to be very stable organelles, depends on the turnover of key components, suggesting new perspectives for understanding the dysfunction of those structures in disease.\n                </jats:p>","journal":null,"year":null,"id":635699,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":822644,"name":"Tânia Perestrelo","orcid":"0000-0001-8995-6620","position":1,"is_corresponding":false},{"id":1649376,"name":"Patricia Rodrigues","orcid":"0000-0003-1681-508X","position":2,"is_corresponding":false},{"id":694003,"name":"Paulo Duarte","orcid":"0000-0003-1833-5629","position":3,"is_corresponding":false},{"id":1649377,"name":"Mariana Lince-Faria","orcid":"0000-0002-9842-173X","position":4,"is_corresponding":false},{"id":497317,"name":"Mónica Bettencourt‐Dias","orcid":"0000-0003-1987-5598","position":5,"is_corresponding":false},{"id":1649375,"name":"Ana Pimenta-Marques","orcid":"0000-0001-6318-3922","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Centrioles play critical roles in our cells, being part of centrosomes and cilia, which are important microtubule organising centers (MTOC) with a variety of roles. While centrioles are very stable structures, they disappear in certain cell types upon differentiation, such as in oocytes. Little is known about the regulation of centriole structural integrity. We previously uncovered that the pericentriolar material (PCM), and its recruiter Polo kinase, are required for both the maintenance of centriole structural integrity and centrosome MTOC activity. Using an hypothesis driven RNAi screen, we show that both the cartwheel and the centriole wall play an important role in centrosome integrity. In particular, we uncovered that the centriole wall protein ANA1 is critical for the integrity of both new and mature centrioles, in\n                  <jats:italic>Drosophila</jats:italic>\n                  oogenesis as well as in cultured cells. Moreover, our results show that the activity of both Polo and the PCM in centriole integrity depends on ANA1. Our work suggests that the structural integrity of centrioles, once thought to be very stable organelles, depends on the turnover of key components, suggesting new perspectives for understanding the dysfunction of those structures in disease.\n                </jats:p>","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19767382","pmcid":null,"openalex_id":"https://openalex.org/W4226353050","authors":[],"funders":[{"funder_name":"European Commission","grant_id":"683258","title":"Mechanism of centriole inheritance and maintenance"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2023,"count":1}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2022/04/06/2022.04.06.487296.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2022/04/06/2022.04.06.487296.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2022.04.06.487296","host_type":"publisher"},{"url":"https://doi.org/10.1101/2022.04.06.487296","host_type":"repository"},{"url":"http://dx.doi.org/10.1101/2022.04.06.487296","host_type":""}],"fields_of_study":["Microtubule and mitosis dynamics","Genetic and Kidney Cyst Diseases","Photosynthetic Processes and Mechanisms","0301 basic medicine","03 medical and health sciences"],"mesh_terms":[],"keywords":["Centrosome","Centriole","Cell biology","Microtubule organizing center","Biology","Microtubule","Basal body","Cell cycle","Flagellum","Cell","Genetics","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T15:27:17.152188Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}