{"doi":"10.1101/2022.03.31.486548","title":"Antibody evolution to SARS-CoV-2 after single-dose Ad26.COV2.S vaccine","abstract":"Abstract The single dose Ad.26.COV.2 (Janssen) vaccine elicits lower levels of neutralizing antibodies and shows more limited efficacy in protection against infection than either of the available mRNA vaccines. In addition, the Ad.26.COV.2 has been less effective in protection against severe disease during the Omicron surge. Here, we examined the memory B cell response to single dose Ad.26.COV.2 vaccination. Compared to mRNA vaccines, Ad.26.COV.2 recipients had significantly lower numbers of RBD-specific memory B cells 1.5 or 6 months after vaccination. Memory antibodies elicited by both vaccine types show comparable neutralizing potency against SARS-CoV-2 and Delta. However, the number of memory cells producing Omicron neutralizing antibodies was somewhat lower after Ad.26.COV.2 than mRNA vaccination. The data help explain why boosting Ad.26.COV.2 vaccine recipients with mRNA vaccines is effective, and why the Janssen vaccine appears to have been less protective against severe disease during the Omicron surge than the mRNA vaccine. One-Sentence Summary Ad.26.COV.2 vaccine results in lower quantity but comparable quality of protective memory B cells compared to mRNA vaccines.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":303062,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9546,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":16973,"name":"Frauke Muecksch","orcid":"0000-0002-0132-5101","position":1,"is_corresponding":false},{"id":104706,"name":"Zijun Wang","orcid":"0000-0002-2095-2151","position":2,"is_corresponding":false},{"id":619035,"name":"Tarek Ben Tanfous","orcid":"0000-0002-5575-5035","position":3,"is_corresponding":false},{"id":105899,"name":"Justin DaSilva","orcid":"0000-0001-6393-0000","position":4,"is_corresponding":false},{"id":840059,"name":"Raphael Raspe","orcid":"0000-0003-3409-4968","position":5,"is_corresponding":false},{"id":694729,"name":"Brianna Johnson","orcid":"0000-0003-3214-284X","position":6,"is_corresponding":false},{"id":109695,"name":"Eva Bednarski","orcid":"0000-0003-4438-5590","position":7,"is_corresponding":false},{"id":104721,"name":"Víctor Ramos","orcid":"0000-0001-7353-3420","position":8,"is_corresponding":false},{"id":552810,"name":"Dennis Schaefer-Babajew","orcid":"0000-0002-5380-2950","position":9,"is_corresponding":false},{"id":104724,"name":"Irina Shimeliovich","orcid":"0000-0002-7030-294X","position":10,"is_corresponding":false},{"id":104723,"name":"Juan Dizon","orcid":"0000-0003-1956-1169","position":11,"is_corresponding":false},{"id":301424,"name":"Kai-Hui Yao","orcid":"0000-0001-7912-5516","position":12,"is_corresponding":false},{"id":104730,"name":"Fabian Schmidt","orcid":"0000-0001-7731-6685","position":13,"is_corresponding":false},{"id":104715,"name":"Katrina G. Millard","orcid":"0000-0003-2247-3178","position":14,"is_corresponding":false},{"id":552811,"name":"Martina Turroja","orcid":"0000-0001-9640-0559","position":15,"is_corresponding":false},{"id":254283,"name":"Mila Janković","orcid":"0000-0001-9696-5234","position":16,"is_corresponding":false},{"id":104712,"name":"Thiago Y. Oliveira","orcid":"0000-0002-2654-0879","position":17,"is_corresponding":false},{"id":104709,"name":"Anna Gazumyan","orcid":"0000-0002-5976-9717","position":18,"is_corresponding":false},{"id":104704,"name":"Christian Gaebler","orcid":"0000-0001-7295-8128","position":19,"is_corresponding":false},{"id":104739,"name":"Marina Caskey","orcid":"0000-0003-1727-8693","position":20,"is_corresponding":false},{"id":104738,"name":"Théodora Hatziioannou","orcid":"0000-0002-7889-0766","position":21,"is_corresponding":false},{"id":16992,"name":"Paul D. Bieniasz","orcid":"0000-0002-2368-3719","position":22,"is_corresponding":false},{"id":104187,"name":"Michel C. Nussenzweig","orcid":"0000-0003-0592-8564","position":23,"is_corresponding":false},{"id":104707,"name":"Alice Cho","orcid":"0000-0001-6354-6148","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T00:32:20.348385Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}