{"doi":"10.1101/2022.03.29.486247","title":"Transcriptomics, Regulatory Syntax, and Enhancer Identification in Heterogenous Populations of Mesoderm-Induced ESCs at Single-Cell Resolution","abstract":"SUMMARY ESCs can adopt lineage-specific gene expression programs by stepwise exposure to defined factors, resulting in the generation of functional cell types. Bulk and single cell-based assays were employed to catalogue gene expression, histone modifications, chromatin conformation, and accessibility transitions in ESC populations and individual cells acquiring a presomitic mesoderm fate and undergoing further lineage specification. These assays identified cis -regulatory regions and transcription factors presiding gene expression programs occurring at defined ESC transitions and revealed the presence of heterogeneous cell populations within discrete ESC developmental stages. The datasets were employed to identify previously unappreciated genomic elements directing the initial activation of Pax7 and myogenic and neurogenic gene expression programs. This study provides a resource for the discovery of genomic and transcriptional features of pluripotent, mesoderm-induced ESCs, and ESCs-derived cell lineages.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":302853,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9225,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":482413,"name":"Jelena Perovanović","orcid":"0000-0003-2348-2332","position":1,"is_corresponding":false},{"id":922426,"name":"Kyung Dae Ko","orcid":null,"position":2,"is_corresponding":false},{"id":844373,"name":"Kan Jiang","orcid":"0000-0001-9065-4574","position":3,"is_corresponding":false},{"id":994987,"name":"Xuesong Feng","orcid":"0000-0002-5992-8650","position":4,"is_corresponding":false},{"id":922427,"name":"Natalia Acevedo-Luna","orcid":null,"position":5,"is_corresponding":false},{"id":921991,"name":"Jérome Chal","orcid":"0000-0002-0468-7677","position":6,"is_corresponding":false},{"id":922428,"name":"Veronica Ciuffoli","orcid":null,"position":7,"is_corresponding":false},{"id":395752,"name":"Pavol Genzor","orcid":"0000-0002-3316-5659","position":8,"is_corresponding":false},{"id":921992,"name":"James N. Simone","orcid":"0000-0001-8515-3337","position":9,"is_corresponding":false},{"id":395753,"name":"Astrid D. Haase","orcid":"0000-0003-1120-9013","position":10,"is_corresponding":false},{"id":250294,"name":"Olivier Pourquié","orcid":"0000-0001-5189-1227","position":11,"is_corresponding":false},{"id":218256,"name":"Stefania Dell’Orso","orcid":"0000-0002-1634-5631","position":12,"is_corresponding":false},{"id":230554,"name":"Vittorio Sartorelli","orcid":"0000-0002-9284-3675","position":13,"is_corresponding":false},{"id":921988,"name":"Mamduh Khateb","orcid":"0000-0002-6347-3361","position":0,"is_corresponding":true}],"reference_count":92,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:32:20.348385Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}