{"doi":"10.1101/2022.03.24.485622","title":"Scalable Generation of Pseudo-Unipolar Sensory Neurons from Human Pluripotent Stem Cells","abstract":"ABSTRACT Development of new non-addictive analgesics requires advanced strategies to differentiate human pluripotent stem cells (hPSCs) into relevant cell types amenable for translational research. Here, we developed a highly efficient and reproducible method that differentiates hPSCs into peptidergic and non-peptidergic nociceptors. By modulating specific cell signaling pathways, hPSCs were first converted into SOX10 + neural crest cells, followed by differentiation into sensory neurons with an in vivo -like pseudo-unipolar morphology. Detailed characterization confirmed that the hPSC-derived nociceptors displayed molecular and cellular features comparable to native dorsal root ganglion (DRG) neurons, and expressed high-threshold primary sensory neuron markers, transcription factors, neuropeptides, and over 150 ion channels and receptors, including critical pain-relevant drug targets (e.g., TRPV1, TAC1, CALCA, NA V 1.7, NA V 1.8). Moreover, after confirming robust functional activities and differential response to noxious stimuli and specific drugs, a robotic cell culture system was employed to produce large quantities of human sensory neurons, which can be used to develop nociceptor-selective analgesics.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":299099,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9493,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":561230,"name":"Carlos A. Tristan","orcid":null,"position":1,"is_corresponding":false},{"id":867109,"name":"Claire Weber","orcid":"0000-0002-2352-8479","position":2,"is_corresponding":false},{"id":410303,"name":"Pei‐Hsuan Chu","orcid":null,"position":3,"is_corresponding":false},{"id":625106,"name":"Seungmi Ryu","orcid":"0000-0001-8461-5878","position":4,"is_corresponding":false},{"id":560505,"name":"Vukasin M. Jovanovic","orcid":"0000-0002-0226-1998","position":5,"is_corresponding":false},{"id":560503,"name":"Pinar Ormanoglu","orcid":"0000-0002-2384-8253","position":6,"is_corresponding":false},{"id":988964,"name":"Prisca Twumasi","orcid":null,"position":7,"is_corresponding":false},{"id":407585,"name":"Jaehoon Shim","orcid":"0000-0001-7981-2658","position":8,"is_corresponding":false},{"id":372479,"name":"Selwyn S. Jayakar","orcid":"0000-0002-5792-6408","position":9,"is_corresponding":false},{"id":530364,"name":"Han-Xiong Bear Zhang","orcid":"0000-0003-2327-6694","position":10,"is_corresponding":false},{"id":444107,"name":"Sooyeon Jo","orcid":"0000-0001-5555-6514","position":11,"is_corresponding":false},{"id":473257,"name":"Ty C. Voss","orcid":"0009-0002-8750-2478","position":12,"is_corresponding":false},{"id":228423,"name":"Anton Simeonov","orcid":"0000-0002-4523-9977","position":13,"is_corresponding":false},{"id":444108,"name":"Bruce P. Bean","orcid":"0000-0002-5093-3576","position":14,"is_corresponding":false},{"id":262675,"name":"Clifford J. Woolf","orcid":"0000-0002-6636-3897","position":15,"is_corresponding":false},{"id":560507,"name":"Ilyas Singeç","orcid":"0000-0003-3941-1838","position":16,"is_corresponding":false},{"id":625107,"name":"Tao Deng","orcid":"0000-0002-8303-0891","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":null,"created_at":"2026-07-19T00:31:40.528568Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}