{"doi":"10.1101/2022.03.17.484790","title":"Basic helix-loop-helix pioneer factors interact with the histone octamer to invade nucleosomes and generate nucleosome depleted regions","abstract":"SUMMARY Nucleosomes drastically limit transcription factor (TF) occupancy, while pioneer transcription factors (PFs) somehow circumvent this nucleosome barrier. In this study, we compare nucleosome binding of two conserved S. cerevisiae basic helix-loop-helix (bHLH) TFs, Cbf1 and Pho4. A Cryo-EM structure of Cbf1 in complex with the nucleosome reveals that the Cbf1 HLH region can electrostatically interact with exposed histone residues within a partially unwrapped nucleosome. Single molecule fluorescence studies show that the Cbf1 but not the Pho4 HLH region facilitates efficient nucleosome invasion by slowing its dissociation rate relative to DNA through interactions with histones. In vivo studies show that this enhanced binding provided by the Cbf1 HLH region enables nucleosome invasion and ensuing repositioning. These structural, single molecule, and in vivo studies reveal the mechanistic basis of dissociation rate compensation by pioneer factors and how this translates to facilitating chromatin opening inside cells.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":299974,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9661,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":884731,"name":"Hengye Chen","orcid":"0000-0002-5354-0751","position":1,"is_corresponding":false},{"id":274209,"name":"Priit Eek","orcid":"0000-0003-0702-9466","position":2,"is_corresponding":false},{"id":821741,"name":"Zhiyuan Meng","orcid":"0000-0003-4352-8561","position":3,"is_corresponding":false},{"id":990217,"name":"Caroline Jipa","orcid":"0000-0003-2934-5734","position":4,"is_corresponding":false},{"id":274210,"name":"Song Tan","orcid":"0000-0002-6698-8475","position":5,"is_corresponding":false},{"id":447230,"name":"Lu Bai","orcid":"0000-0003-3667-2944","position":6,"is_corresponding":false},{"id":111546,"name":"Michael G. Poirier","orcid":"0000-0002-1563-5792","position":7,"is_corresponding":false},{"id":932843,"name":"Benjamin Donovan","orcid":"0000-0003-0177-799X","position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":null,"created_at":"2026-07-19T00:31:49.412501Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}