{"doi":"10.1101/2022.02.22.481519","title":"Heat Shock Factor 1 (HSF1) specifically potentiates c-MYC-mediated transcription independently of the canonical heat-shock response","abstract":"ABSTRACT Despite its pivotal roles in biology, how the transcriptional activity of c-MYC is attuned quantitatively remain poorly defined. Here, we show that heat shock factor 1 (HSF1), the master transcriptional regulator of the heat-shock, or proteotoxic stress, response, acts as a key modifier of the c-MYC-mediated transcription. HSF1 deficiency diminishes c-MYC DNA binding and dampens its transcriptional activity genome-widely. Mechanistically, c-MYC, MAX, and HSF1 assemble into a transcription factor complex on genomic DNAs and, surprisingly, the DNA binding of HSF1 is dispensable. Instead, HSF1 physically recruits the histone acetyltransferase GCN5, thereby promoting histone acetylation and augmenting c-MYC transcriptional activity. Thus, our studies reveal that HSF1 specifically potentiates the c-MYC-mediated transcription, distinct from its role in the canonical heat-shock response. Importantly, this mechanism of action engenders two distinct c-MYC activation states, primary and advanced, which may be important to accommodate diverse physiological and pathological conditions.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":306096,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9605,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":746909,"name":"Ling Lin","orcid":"0000-0001-5527-0575","position":1,"is_corresponding":false},{"id":1000031,"name":"Kun‐Han Chuang","orcid":"0000-0003-3241-8586","position":2,"is_corresponding":false},{"id":1000032,"name":"Babul Moni Ram","orcid":"0000-0003-4440-8801","position":3,"is_corresponding":false},{"id":895993,"name":"Siyuan Dai","orcid":"0000-0002-4062-5211","position":4,"is_corresponding":false},{"id":482308,"name":"Kuo‐Hui Su","orcid":"0000-0002-0834-8568","position":5,"is_corresponding":false},{"id":482307,"name":"Zijian Tang","orcid":"0000-0002-0888-9217","position":6,"is_corresponding":false},{"id":482310,"name":"Chengkai Dai","orcid":"0000-0001-8520-1036","position":7,"is_corresponding":false},{"id":482309,"name":"Meng Xu","orcid":"0000-0001-7387-0734","position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":null,"created_at":"2026-07-19T00:32:44.983506Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}