{"doi":"10.1101/2022.02.17.480942","title":"Single-Cell Multi-Omic Roadmap of Human Fetal Pancreatic Development","abstract":"ABSTRACT The critical cellular transitions that govern human pancreas development are largely unknown. We performed large-scale single-cell RNA-sequencing (scRNA-Seq) to interrogate human fetal pancreas development from 8-20 weeks post conception. We identified 103 distinct cell types, including four novel endocrine progenitor subtypes displaying unique transcriptional features and differentiation potency. Integration with single-nucleus Assay for Transposase Accessible Chromatin Sequencing (snATAC-Seq) identified candidate regulators of human endocrine cell fate and revealed development-specific regulatory annotation at diabetes risk loci. Comparison of in vitro stem cell-derived and endogenous endocrine cells predicted aberrant genetic programs leading to the generation of off-target cells. Finally, knock-out studies revealed that the gene FEV regulates human endocrine differentiation. This work establishes a roadmap of human pancreatic development, highlights previously unappreciated cellular diversity and lineage dynamics, and provides a blueprint for understanding pancreatic disease and physiology, as well as generating human stem cell-derived islet cells in vitro for regenerative medicine purposes.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":296539,"datarank":0.8370907208530425,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.3219926401802705,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.3219926401802705,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":24,"citers_with_citation_signal":17,"citers_with_endowment":17,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.5049,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":758298,"name":"Zhe Liu","orcid":"0000-0003-0726-2727","position":1,"is_corresponding":false},{"id":90170,"name":"Han Sun","orcid":"0000-0002-3390-0027","position":2,"is_corresponding":false},{"id":983888,"name":"Shengyang K. Yu","orcid":null,"position":3,"is_corresponding":false},{"id":98294,"name":"Daniel M. Wong","orcid":"0000-0002-2663-0153","position":4,"is_corresponding":false},{"id":908989,"name":"Emily Chu","orcid":"0009-0008-1508-504X","position":5,"is_corresponding":false},{"id":759011,"name":"Sneha A. Rao","orcid":null,"position":6,"is_corresponding":false},{"id":890910,"name":"Nicolas Eng","orcid":null,"position":7,"is_corresponding":false},{"id":983889,"name":"Gabriel A. Peixoto","orcid":null,"position":8,"is_corresponding":false},{"id":983890,"name":"Jacquelyn Bouza","orcid":null,"position":9,"is_corresponding":false},{"id":983358,"name":"Yin Shen","orcid":"0000-0001-8380-2428","position":10,"is_corresponding":false},{"id":98297,"name":"Sarah M. Knox","orcid":"0000-0002-7567-083X","position":11,"is_corresponding":false},{"id":79461,"name":"Aaron D. Tward","orcid":"0000-0003-4868-8732","position":12,"is_corresponding":false},{"id":7383,"name":"Anna L. Gloyn","orcid":"0000-0003-1205-1844","position":13,"is_corresponding":false},{"id":15872,"name":"Julie B. Sneddon","orcid":"0000-0002-7404-8598","position":14,"is_corresponding":false},{"id":15864,"name":"Sean de la O","orcid":"0000-0001-6607-9610","position":0,"is_corresponding":true}],"reference_count":126,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:31:16.555318Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}