{"doi":"10.1101/2022.02.14.480453","title":"Microbiota-derived aspartate drives pathogenic Enterobacteriaceae expansion in the inflamed gut","abstract":"SUMMARY Inflammation boosts the availability of electron acceptors in the intestinal lumen creating a favorable niche for pathogenic Enterobacteriaceae. However, the mechanisms linking intestinal inflammation-mediated changes in luminal metabolites and pathogen expansion remain unclear. Here, we show that mucosal inflammation induced by Salmonella enterica serovar Typhimurium ( S. Tm) infection and chemical colitis results in increased intestinal levels of the amino acid aspartate. The S. Tm and E. coli genomes encode an aspartate ammonia-lyase ( aspA ) which converts aspartate into fumarate, an alternative electron acceptor. S. Tm and pathogenic E. coli used aspA- dependent fumarate respiration for growth in the murine gut only during inflammation. Such growth advantage was abolished in the gut of germ-free mice. However, mono-association of gnotobiotic mice with members of the classes Bacteroidia and Clostridia restored the benefit of aspartate utilization to the pathogens. Our findings demonstrate the role of microbiota-derived amino acids in driving respiration-dependent Enterobacteriaceae expansion during colitis.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":299843,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9545,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":856006,"name":"Jacob K. Zieba","orcid":"0000-0003-4163-6569","position":1,"is_corresponding":false},{"id":663149,"name":"Nicolas G. Shealy","orcid":"0000-0002-1700-7570","position":2,"is_corresponding":false},{"id":856005,"name":"Teresa P. Torres","orcid":"0000-0003-2316-4705","position":3,"is_corresponding":false},{"id":990471,"name":"Julia Thomas","orcid":null,"position":4,"is_corresponding":false},{"id":631455,"name":"Catherine Shelton","orcid":null,"position":5,"is_corresponding":false},{"id":412130,"name":"Nora J. Foegeding","orcid":"0000-0002-4197-9249","position":6,"is_corresponding":false},{"id":630396,"name":"Erin E. Olsan","orcid":"0000-0002-5650-1133","position":7,"is_corresponding":false},{"id":366399,"name":"Mariana X. Byndloss","orcid":"0000-0002-2122-4156","position":8,"is_corresponding":false},{"id":663150,"name":"Woongjae Yoo","orcid":"0000-0002-4139-8217","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-19T00:31:49.412501Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}