{"doi":"10.1101/2022.02.11.480005","title":"Exponential magnetophoretic gradient for the direct isolation of basophils from whole blood in a microfluidic system","abstract":"Abstract Despite their rarity in peripheral blood, basophils play important roles in allergic disorders and other diseases including sepsis and COVID-19. Existing basophil isolation methods require many manual steps and suffer from significant variability in purity and recovery. We report an integrated basophil isolation device (i-BID) in microfluidics for negative immunomagnetic selection of basophils directly from 100 μL of whole blood within 10 minutes. We use a simulation-driven pipeline to design a magnetic separation module to apply an exponentially increasing magnetic force to capture magnetically tagged non-basophils flowing through a microtubing sandwiched between magnetic flux concentrators sweeping across a Halbach array. The exponential profile captures non-basophils effectively while preventing their excessive initial buildup causing clogging. The i-BID isolates basophils with a mean purity of 93.9%±3.6% and recovery of 95.6%±3.4% without causing basophil degradation or unintentional activation. Our i-BID has the potential to enable basophil-based point-of-care diagnostics such as rapid allergy assessment.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":302671,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9525,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":994592,"name":"Sun-Gu Kim","orcid":"0000-0001-7446-1123","position":1,"is_corresponding":false},{"id":994593,"name":"Adrian M. Martin","orcid":"0000-0003-0078-2439","position":2,"is_corresponding":false},{"id":73046,"name":"Stephen J. Galli","orcid":"0000-0001-5736-5340","position":3,"is_corresponding":false},{"id":36438,"name":"Kari C. Nadeau","orcid":"0000-0002-2146-2955","position":4,"is_corresponding":false},{"id":469531,"name":"Sindy K. Y. Tang","orcid":"0000-0003-1465-2432","position":5,"is_corresponding":false},{"id":994591,"name":"Nicolas Castaño","orcid":"0000-0002-9244-3313","position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":null,"created_at":"2026-07-19T00:32:16.279991Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}