{"doi":"10.1101/2022.01.28.478036","title":"Sex-specific regulation of binge drinking, social, and arousal behaviors by subcortical serotonin 5HT <sub>2c</sub> receptor-containing neurons","abstract":"Summary Serotonin 5HT 2c receptors have been implicated in the pathophysiology of both mood disorders and alcohol use disorder, but the circuits mediating the effects of systemic pharmacological manipulations of this receptor on behavior have not been identified. Binge alcohol consumption induces discrete social and arousal disturbances in human populations, which are thought to promote increased drinking. However, whether models of binge drinking in rodents can induce these same long-term negative behavioral symptoms is unknown. In this study, we employed multiple anatomical, physiological, and behavioral approaches to identify two populations of neurons expressing serotonin 5HT 2c receptors, one in the lateral habenula (LHb 5HT2c ) and one in the bed nucleus of the stria terminalis (BNST 5HT2c ), that display coordinated in-vivo responses to social, arousal, and alcohol-related stimuli and are physiologically modulated by binge alcohol consumption in a sex-specific manner. Critically, these physiological changes were associated with sex-specific behavioral disturbances that closely mirror social and arousal symptoms observed in humans during abstinence from binge drinking. Specifically, we observed that one week of abstinence from three weeks of binge alcohol drinking induced social recognition deficits in females and potentiated acoustic startle responses in males. While both populations of neurons (BNST and LHb) and the 5HT 2c receptor itself contribute to the sex-specific effects of alcohol on social and arousal behaviors to some degree, the primary causal mechanism underlying these phenomena appears to be excessive activation of LHb 5HT2c neurons. These findings may have implications for the development of sex-specific treatments for mood and alcohol use disorders targeting the brain’s serotonin system.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":302705,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.958,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":249811,"name":"Olivia J. Hon","orcid":"0000-0003-1086-1421","position":1,"is_corresponding":false},{"id":368538,"name":"Shannon D’Ambrosio","orcid":"0000-0002-2129-4728","position":2,"is_corresponding":false},{"id":343649,"name":"Kristen M. Boyt","orcid":null,"position":3,"is_corresponding":false},{"id":994906,"name":"Leslie A. Hassanein","orcid":"0000-0002-6332-6070","position":4,"is_corresponding":false},{"id":995206,"name":"M. Castle","orcid":null,"position":5,"is_corresponding":false},{"id":477470,"name":"Harold L. Haun","orcid":"0000-0002-4781-3733","position":6,"is_corresponding":false},{"id":818278,"name":"Melanie M. Pina","orcid":"0000-0002-5638-0474","position":7,"is_corresponding":false},{"id":249826,"name":"Thomas L. Kash","orcid":"0000-0002-4747-4495","position":8,"is_corresponding":false},{"id":399230,"name":"Meghan E. Flanigan","orcid":"0000-0002-3185-7459","position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":null,"created_at":"2026-07-19T00:32:20.348385Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}