{"doi":"10.1101/2022.01.24.477592","title":"Identification of putative reader proteins of 5-methylcytosine and its derivatives in <i>Caenorhabditis elegans</i> RNA","abstract":"ABSTRACT Methylation of carbon-5 of cytosines (m 5 C) is a post-transcriptional nucleotide modification of RNA with widespread distribution across organisms. m 5 C has been shown to participate in mRNA transport and maintain mRNA stability through its recognition by the reader proteins ALYREF and YBX1, respectively. We recently showed that m 5 C is required for Caenorhabditis elegans development and fertility under heat stress. To contribute to the understanding of how m 5 C and its oxidative derivatives mediate their functions, we developed RNA baits bearing modified cytosines in diverse structural contexts to pulldown potential readers in C. elegans . Our mass spectrometry analyses reveal unique binding proteins for each of the modifications. We validate our dataset by demonstrating that the nematode ALYREF homologues ALY-1 and ALY-2 preferentially bind m 5 C in vitro . The dataset presented here serves as an important scientific resource that will support the discovery of new functions of m 5 C and its derivatives.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":305595,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9566,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":489044,"name":"Kin Man Suen","orcid":"0000-0001-9452-2528","position":1,"is_corresponding":false},{"id":489047,"name":"Dalila Bensaddek","orcid":"0000-0002-5995-770X","position":2,"is_corresponding":false},{"id":974847,"name":"Arun A. Tanpure","orcid":"0000-0002-6232-740X","position":3,"is_corresponding":false},{"id":65596,"name":"Angus I. Lamond","orcid":"0000-0001-6204-6045","position":4,"is_corresponding":false},{"id":94616,"name":"Shankar Balasubramanian","orcid":"0000-0002-0281-5815","position":5,"is_corresponding":false},{"id":304354,"name":"Eric A. Miska","orcid":"0000-0002-4450-576X","position":6,"is_corresponding":false},{"id":974846,"name":"Isabela Cunha Navarro","orcid":"0000-0002-6844-2361","position":0,"is_corresponding":true}],"reference_count":34,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:32:41.290883Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}