{"doi":"10.1101/2022.01.17.476679","title":"Development of an HIV reporter virus that identifies latently infected CD4 <sup>+</sup> T cells","abstract":"SUMMARY There is no cure for HIV infection as the virus establishes a latent reservoir, which escapes highly active antiretroviral treatments. One major obstacle is the difficulty to identify cells that harbor latent proviruses. We devised a novel viral vector that carries a series of versatile reporter molecules that are expressed in an LTR-dependent or LTR-independent manner and allows to accurately distinguish productive from latent infection. Using primary human CD4 + T cells, we show that transcriptionally silent proviruses make up over 50% of all infected cells. These latently infected cells harbor proviruses, but lack evidence for viral transcription. LTR silent integrations occurred to variable degrees in all CD4 + T-subsets examined with CD4 + T EM and CD4 + T reg displaying the highest frequency. Viral vectors such as the one described here, permit interrogation HIV latency at a single-cell resolution, revealing mechanisms of latency establishment and allowing for the characterization of effective latency reversing agents.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":302450,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":887265,"name":"Lara Manganaro","orcid":"0000-0003-2743-2658","position":1,"is_corresponding":false},{"id":107923,"name":"Michael Schotsaert","orcid":"0000-0003-3156-3132","position":2,"is_corresponding":false},{"id":105231,"name":"Brian D. Brown","orcid":"0000-0002-3670-8778","position":3,"is_corresponding":false},{"id":617071,"name":"Lubbertus C. F. Mulder","orcid":null,"position":4,"is_corresponding":false},{"id":104936,"name":"Viviana Simon","orcid":"0000-0002-6416-5096","position":5,"is_corresponding":false},{"id":994451,"name":"Eun Hye Kim","orcid":"0000-0001-6691-6837","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-19T00:32:16.279991Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}