{"doi":"10.1101/2022.01.14.476215","title":"B cells discriminate HIV-1 Envelope protein affinities by sensing antigen binding association rates","abstract":"SUMMARY HIV-1 Envelope (Env) proteins designed to induce neutralizing antibody responses allow study of the role of affinities (equilibrium dissociation constant, K D ) and kinetic rates (association/dissociation rates) on B cell antigen recognition. It is unclear whether affinity discrimination during B cell activation is based solely on Env protein binding K D , and whether B cells discriminate between proteins of similar affinities but that bind with different kinetic rates. Here we used a panel of Env proteins and Ramos B cell lines expressing IgM BCRs with specificity for CD4 binding-site broadly neutralizing (bnAb) or a precursor antibody to study the role of antigen binding kinetic rates on both early (proximal/distal signaling) and late events (BCR/antigen internalization) in B cell activation. Our results support a kinetic model for B cell activation in which Env protein affinity discrimination is based not on overall K D , but on sensing of association rate and a threshold antigen-BCR half-life.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":305391,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9584,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":601089,"name":"Kara Anasti","orcid":"0000-0002-1854-743X","position":1,"is_corresponding":false},{"id":642735,"name":"Brian Watts","orcid":"0000-0003-1731-2441","position":2,"is_corresponding":false},{"id":444168,"name":"Kenneth Cronin","orcid":"0000-0002-5984-5978","position":3,"is_corresponding":false},{"id":913725,"name":"Advaiti Pai Kane","orcid":null,"position":4,"is_corresponding":false},{"id":227358,"name":"Robert J. Edwards","orcid":"0000-0003-4446-1194","position":5,"is_corresponding":false},{"id":392395,"name":"David Easterhoff","orcid":"0000-0001-5223-3277","position":6,"is_corresponding":false},{"id":724904,"name":"Jinsong Zhang","orcid":"0000-0002-2195-2997","position":7,"is_corresponding":false},{"id":343910,"name":"Wes Rountree","orcid":"0000-0001-5183-6949","position":8,"is_corresponding":false},{"id":913727,"name":"Yaneth Ortiz","orcid":null,"position":9,"is_corresponding":false},{"id":469215,"name":"Laurent Verkoczy","orcid":"0000-0001-7497-6645","position":10,"is_corresponding":false},{"id":851771,"name":"Michael Reth","orcid":"0000-0002-1025-7198","position":11,"is_corresponding":false},{"id":258350,"name":"S. Munir Alam","orcid":"0000-0003-0941-0703","position":12,"is_corresponding":false},{"id":533616,"name":"Md. Alamgir Hossain","orcid":"0000-0003-4978-1664","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":null,"created_at":"2026-07-19T00:32:41.290883Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}