{"doi":"10.1101/2022.01.06.475243","title":"Multi-micron crisscross structures from combinatorially assembled DNA-origami slats","abstract":"Abstract Living systems achieve robust self-assembly across length scales. Meanwhile, nanofabrication strategies such as DNA origami have enabled robust self-assembly of submicron-scale shapes.However, erroneous and missing linkages restrict the number of unique origami that can be practically combined into a single supershape. We introduce crisscross polymerization of DNA-origami slats for strictly seed-dependent growth of custom multi-micron shapes with user-defined nanoscale surface patterning. Using a library of ~2000 strands that can be combinatorially assembled to yield any of ~1e48 distinct DNA origami slats, we realize five-gigadalton structures composed of >1000 uniquely addressable slats, and periodic structures incorporating >10,000 slats. Thus crisscross growth provides a generalizable route for prototyping and scalable production of devices integrating thousands of unique components that each are sophisticated and molecularly precise. One-sentence summary Crisscross polymerization of DNA-origami slats can yield micron-scale structures with uniquely addressable nanoscale features.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":297319,"datarank":0.3596842909197557,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.0,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9498,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":985951,"name":"Dionis Minev","orcid":null,"position":1,"is_corresponding":false},{"id":985328,"name":"Anastasia Ershova","orcid":"0000-0002-7798-615X","position":2,"is_corresponding":false},{"id":985329,"name":"Hiroshi Sasaki","orcid":"0000-0001-9201-8766","position":3,"is_corresponding":false},{"id":985330,"name":"Gokul Gowri","orcid":"0000-0003-0911-6889","position":4,"is_corresponding":false},{"id":985331,"name":"Jonathan F. Berengut","orcid":"0000-0002-6802-6763","position":5,"is_corresponding":false},{"id":985332,"name":"F. Eduardo Corea-Dilbert","orcid":"0000-0002-1779-4922","position":6,"is_corresponding":false},{"id":44857,"name":"Peng Yin","orcid":"0000-0002-2769-6357","position":7,"is_corresponding":false},{"id":478689,"name":"William M. Shih","orcid":"0000-0002-1395-9267","position":8,"is_corresponding":false},{"id":985327,"name":"Christopher M. Wintersinger","orcid":"0000-0003-4633-9651","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:31:26.161109Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}