{"doi":"10.1101/2021.12.28.474244","title":"Design, immunogenicity, and efficacy of a pan-sarbecovirus dendritic-cell targeting vaccine","abstract":"Abstract The emergence of SARS-CoV-2 variants of concern (VOCs) that escape pre-existing antibody neutralizing responses increases the need for vaccines that target conserved epitopes and induce cross-reactive B- and T-cell responses. We used a computational approach and sequence alignment analysis to design a new-generation subunit vaccine targeting conserved sarbecovirus B- and T-cell epitopes from Spike (S) and Nucleocapsid (N) to antigen-presenting cells expressing CD40 (CD40.CoV2). We demonstrate the potency of CD40.CoV2 to elicit high levels of cross-neutralizing antibodies against SARS-CoV-2, VOCs, and SARS-CoV-1 in K18-hACE2 transgenic mice, associated with improved viral control and survival after challenge. In addition, we demonstrate the potency of CD40.CoV2 in vitro to recall human multi-epitope, functional, and cytotoxic SARS-CoV-2 S- and N-specific T-cell responses that are unaffected by VOC mutations and cross-reactive with SARS-CoV-1 and, to a lesser extent, MERS epitopes. Overall, these findings provide a framework for a pan-sarbecovirus vaccine.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":228549,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9523,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":833599,"name":"Aurélie Wiedemann","orcid":"0000-0002-4224-3137","position":1,"is_corresponding":false},{"id":729734,"name":"Mathieu Surénaud","orcid":"0000-0002-3312-7871","position":2,"is_corresponding":false},{"id":729741,"name":"Christine Lacabaratz","orcid":"0000-0001-6925-0225","position":3,"is_corresponding":false},{"id":833600,"name":"Sophie Hüe","orcid":"0000-0002-2530-7346","position":4,"is_corresponding":false},{"id":833601,"name":"Mélanie Prague","orcid":"0000-0001-9809-7848","position":5,"is_corresponding":false},{"id":833602,"name":"Minerva Cervantes-Gonzalez","orcid":"0000-0003-2813-2219","position":6,"is_corresponding":false},{"id":833603,"name":"Zhiqing Wang","orcid":"0000-0003-3189-2630","position":7,"is_corresponding":false},{"id":833604,"name":"Jerome Ellis","orcid":"0000-0001-5224-3889","position":8,"is_corresponding":false},{"id":833813,"name":"Amandine Sansoni","orcid":null,"position":9,"is_corresponding":false},{"id":833814,"name":"Camille Pierini","orcid":null,"position":10,"is_corresponding":false},{"id":833815,"name":"Quentin Bardin","orcid":null,"position":11,"is_corresponding":false},{"id":833816,"name":"Manon Fabregue","orcid":null,"position":12,"is_corresponding":false},{"id":833817,"name":"Sarah Sharkaoui","orcid":null,"position":13,"is_corresponding":false},{"id":833818,"name":"Philippe Hoest","orcid":null,"position":14,"is_corresponding":false},{"id":833819,"name":"Léa Dupaty","orcid":null,"position":15,"is_corresponding":false},{"id":109850,"name":"Florence Picard","orcid":"0009-0009-1540-4386","position":16,"is_corresponding":false},{"id":833605,"name":"Mireille Centlivre","orcid":"0000-0001-7616-9775","position":17,"is_corresponding":false},{"id":104215,"name":"Jade Ghosn","orcid":"0000-0003-2914-959X","position":18,"is_corresponding":false},{"id":104219,"name":"French COVID Cohort Study Group","orcid":null,"position":19,"is_corresponding":false},{"id":646420,"name":"Rodolphe Thiébaut","orcid":"0000-0002-5235-3962","position":20,"is_corresponding":false},{"id":729745,"name":"Sylvain Cardinaud","orcid":"0000-0002-2650-1108","position":21,"is_corresponding":false},{"id":267607,"name":"Bernard MALISSEN","orcid":"0000-0003-1340-9342","position":22,"is_corresponding":false},{"id":724110,"name":"Gérard Zurawski","orcid":"0000-0002-0168-3523","position":23,"is_corresponding":false},{"id":833606,"name":"Ana Zarubica","orcid":"0000-0001-5488-4137","position":24,"is_corresponding":false},{"id":677370,"name":"Sandra Zurawski","orcid":null,"position":25,"is_corresponding":false},{"id":729743,"name":"Véronique Godot","orcid":"0000-0003-3557-308X","position":26,"is_corresponding":false},{"id":724111,"name":"Yves Lévy","orcid":"0000-0002-5549-6256","position":27,"is_corresponding":false},{"id":833598,"name":"Séverin Coléon","orcid":"0000-0001-5900-2201","position":0,"is_corresponding":true}],"reference_count":91,"raw_metadata":null,"created_at":"2026-07-18T23:54:54.016932Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}