{"doi":"10.1101/2021.12.09.471821","title":"eIF5B and eIF1A remodel human translation initiation complexes to mediate ribosomal subunit joining","abstract":"ABSTRACT Joining of the ribosomal subunits at a translation start site on a messenger RNA during initiation commits the ribosome to synthesize a protein. Here, we combined single-molecule spectroscopy and structural methods using an in vitro reconstituted system to examine how the human ribosomal subunits join. Single-molecule fluorescence revealed when universally-conserved eukaryotic initiation factors (eIFs) eIF1A and eIF5B associate with and depart from initiation complexes. Guided by single-molecule dynamics, we examined initiation complexes that contained both eIF1A and eIF5B using single-particle electron cryo-microscopy. The resulting structure illuminated how eukaryote-specific contacts between eIF1A and eIF5B remodel the initiation complex to orient initiator tRNA in a conformation compatible with ribosomal subunit joining. Collectively, our findings provide a quantitative and architectural framework for the molecular choreography orchestrated by eIF1A and eIF5B during human translation initiation.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":216988,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9576,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":552902,"name":"Rosslyn Grosely","orcid":null,"position":1,"is_corresponding":false},{"id":236134,"name":"Masaaki Sokabe","orcid":"0000-0002-1824-4818","position":2,"is_corresponding":false},{"id":813472,"name":"Carlos Alvarado","orcid":"0000-0002-4570-9211","position":3,"is_corresponding":false},{"id":463182,"name":"Jinfan Wang","orcid":"0000-0003-4170-9289","position":4,"is_corresponding":false},{"id":693237,"name":"Elizabeth Montabana","orcid":null,"position":5,"is_corresponding":false},{"id":813473,"name":"Nancy Villa","orcid":"0009-0007-7095-1468","position":6,"is_corresponding":false},{"id":464462,"name":"Byung‐Sik Shin","orcid":null,"position":7,"is_corresponding":false},{"id":282078,"name":"Thomas Dever","orcid":"0000-0001-7120-9678","position":8,"is_corresponding":false},{"id":236138,"name":"Christopher S. Fraser","orcid":"0000-0001-9626-7743","position":9,"is_corresponding":false},{"id":195162,"name":"Israel S Fernández","orcid":"0000-0001-7218-1603","position":10,"is_corresponding":false},{"id":319748,"name":"Joseph D. Puglisi","orcid":"0000-0001-9268-5112","position":11,"is_corresponding":false},{"id":441322,"name":"Christopher P. Lapointe","orcid":"0000-0002-0406-105X","position":0,"is_corresponding":true}],"reference_count":65,"raw_metadata":null,"created_at":"2026-07-18T23:53:15.868165Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}