{"doi":"10.1101/2021.11.26.470074","title":"Quantitative evaluation of\n                  <i>Mycobacterium abscessus</i>\n                  clinical isolate virulence using a silkworm infection model","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  <jats:italic>Mycobacterium abscessus</jats:italic>\n                  causes chronic skin infections, lung diseases, and systemic or disseminated infections. Here we investigated whether the virulence of\n                  <jats:italic>M. abscessus</jats:italic>\n                  clinical isolates could be evaluated by calculating the median lethal dose (LD\n                  <jats:sub>50</jats:sub>\n                  ) in a silkworm infection model.\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  cells were injected into the silkworm hemolymph. When reared at 37°C, the silkworms died within 2 days post-infection with\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  . Viable cell numbers of\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  increased in the hemolymph of silkworms injected with\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  . Silkworms were not killed by injections with heat-killed\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  cells. The administration of clarithromycin, an antibacterial drug used to treat the infection, prolonged the survival time of silkworms injected with\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  . The LD\n                  <jats:sub>50</jats:sub>\n                  values of 7 clinical isolates were determined using the silkworm infection model and differed by up to 9-fold. The Mb-17 isolate, which was identified as a pathogenic strain in the silkworm infection model, induced more detachment of human THP-1-derived macrophages during infection than the Mb-10 isolate. These findings suggest that the silkworm\n                  <jats:italic>M. abscessus</jats:italic>\n                  infection model with can be used to quantitatively evaluate the virulence of\n                  <jats:italic>M. abscessus</jats:italic>\n                  clinical isolates in a short time period.\n                </jats:p>","journal":null,"year":null,"id":616963,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1590834,"name":"Hanako Fukano","orcid":null,"position":1,"is_corresponding":false},{"id":1415695,"name":"Naoki Hasegawa","orcid":null,"position":2,"is_corresponding":false},{"id":1263037,"name":"Yoshihiko Hoshino","orcid":"0000-0002-3132-0715","position":3,"is_corresponding":false},{"id":1018619,"name":"Takashi Sugita","orcid":"0000-0002-2127-5017","position":4,"is_corresponding":false},{"id":1386828,"name":"Yasuhiko Matsumoto","orcid":"0000-0002-0796-2304","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Quantitative evaluation of\n                  <i>Mycobacterium abscessus</i>\n                  clinical isolate virulence using a silkworm infection model","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  <jats:italic>Mycobacterium abscessus</jats:italic>\n                  causes chronic skin infections, lung diseases, and systemic or disseminated infections. Here we investigated whether the virulence of\n                  <jats:italic>M. abscessus</jats:italic>\n                  clinical isolates could be evaluated by calculating the median lethal dose (LD\n                  <jats:sub>50</jats:sub>\n                  ) in a silkworm infection model.\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  cells were injected into the silkworm hemolymph. When reared at 37°C, the silkworms died within 2 days post-infection with\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  . Viable cell numbers of\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  increased in the hemolymph of silkworms injected with\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  . Silkworms were not killed by injections with heat-killed\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  cells. The administration of clarithromycin, an antibacterial drug used to treat the infection, prolonged the survival time of silkworms injected with\n                  <jats:italic>M. abscessus</jats:italic>\n                  subsp.\n                  <jats:italic>abscessus</jats:italic>\n                  . The LD\n                  <jats:sub>50</jats:sub>\n                  values of 7 clinical isolates were determined using the silkworm infection model and differed by up to 9-fold. The Mb-17 isolate, which was identified as a pathogenic strain in the silkworm infection model, induced more detachment of human THP-1-derived macrophages during infection than the Mb-10 isolate. These findings suggest that the silkworm\n                  <jats:italic>M. abscessus</jats:italic>\n                  infection model with can be used to quantitatively evaluate the virulence of\n                  <jats:italic>M. abscessus</jats:italic>\n                  clinical isolates in a short time period.\n                </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19910364","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":"cc-by","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2022/08/17/2021.11.26.470074.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2021.11.26.470074","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":[],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T00:30:02.236189Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}