{"doi":"10.1101/2021.11.21.469320","title":"Laser Ablation and Fluid Flows Show a Single Force Mechanism Governs Spindle Positioning","abstract":"ABSTRACT Few techniques are available for elucidating the nature of forces that drive subcellular behaviors. Here we develop two complementary ones: 1) femtosecond stereotactic laser ablation (FESLA), which rapidly creates complex cuts of subcellular structures, thereby allowing precise dissection of when, where, and in what direction forces are generated; and 2) assessment of subcellular fluid flows, by comparing direct flow measurements, using microinjected fluorescent nanodiamonds, to large-scale fluid-structure simulations of different models of force transduction. We apply these to study centrosomes in Caenorhabditis elegans early embryos, and use the data to construct a biophysically-based model of centrosome dynamics. Taken together, we demonstrate that cortical pulling forces provide a general explanation for many behaviors mediated by centrosomes, including pronuclear migration/centration and rotation, metaphase spindle positioning, asymmetric spindle elongation and spindle oscillations. In sum, this work establishes new methodologies for disentangling the forces responsible for cell biological phenomena.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":216947,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9557,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":813394,"name":"Gökberk Kabacaoğlu","orcid":"0000-0002-5574-1629","position":1,"is_corresponding":false},{"id":813395,"name":"Ehssan Nazockdast","orcid":"0000-0002-3811-0067","position":2,"is_corresponding":false},{"id":813396,"name":"Huan‐Cheng Chang","orcid":"0000-0002-3515-4128","position":3,"is_corresponding":false},{"id":459733,"name":"Michael Shelley","orcid":"0000-0002-4835-0339","position":4,"is_corresponding":false},{"id":445491,"name":"Daniel Needleman","orcid":"0000-0003-3642-9864","position":5,"is_corresponding":false},{"id":813393,"name":"Hai‐Yin Wu","orcid":"0000-0001-8123-069X","position":0,"is_corresponding":true}],"reference_count":72,"raw_metadata":null,"created_at":"2026-07-18T23:53:15.868165Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}