{"doi":"10.1101/2021.11.12.468419","title":"Human NOP2/NSUN1 Regulates Ribosome Biogenesis Through Non-Catalytic Complex Formation with Box C/D snoRNPs","abstract":"ABSTRACT 5-Methylcytosine (m 5 C) is a base modification broadly found on various RNAs in the human transcriptome. In eukaryotes, m 5 C is catalyzed by enzymes of the NSUN family composed of seven human members (NSUN1-7). NOP2/NSUN1 has been primarily characterized in budding yeast as an essential ribosome biogenesis factor required for the deposition of m 5 C on the 25S ribosomal RNA (rRNA). Although human NOP2/NSUN1 has been known to be an oncogene overexpressed in several types of cancer, its functions and substrates remain poorly characterized. Here we used a miCLIP-seq approach to identify human NOP2/NSUN1 RNA substrates. Our analysis revealed that NOP2/NSUN1 catalyzes the deposition of m 5 C at position 4447 on the 28S rRNA. We also find that NOP2/NSUN1 binds to the 5’ETS region of the pre-rRNA transcript and regulates pre-rRNA processing through non-catalytic complex formation with box C/D snoRNAs. We provide evidence that NOP2/NSUN1 facilitates the recruitment of U3 and U8 snoRNAs to pre-90S ribosomal particles and their stable assembly into snoRNP complexes. Remarkably, expression of both WT and catalytically inactive NOP2/NSUN1 in knockdown background rescues the rRNA processing defects and the stable assembly of box C/D snoRNP complexes, suggesting that NOP2/NSUN1-mediated deposition of m 5 C on rRNA is not required for ribosome synthesis.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":216190,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9518,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":812067,"name":"Anushri Gaur","orcid":null,"position":1,"is_corresponding":false},{"id":812068,"name":"Hunter McConie","orcid":null,"position":2,"is_corresponding":false},{"id":811670,"name":"Amirtha Shekar","orcid":"0000-0003-2449-743X","position":3,"is_corresponding":false},{"id":578046,"name":"Karen Wang","orcid":null,"position":4,"is_corresponding":false},{"id":237237,"name":"Jeffrey T. Chang","orcid":"0000-0002-4578-5636","position":5,"is_corresponding":false},{"id":608941,"name":"Ghislaı̀n Breton","orcid":"0000-0001-6400-3858","position":6,"is_corresponding":false},{"id":811671,"name":"Catherine Denicourt","orcid":"0000-0001-8721-0163","position":7,"is_corresponding":false},{"id":811669,"name":"Han Liao","orcid":"0009-0006-0081-5340","position":0,"is_corresponding":true}],"reference_count":112,"raw_metadata":null,"created_at":"2026-07-18T23:53:00.192353Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}