{"doi":"10.1101/2021.11.08.467793","title":"Fast and sensitive GCaMP calcium indicators for imaging neural populations","abstract":"Abstract Calcium imaging with protein-based indicators is widely used to follow neural activity in intact nervous systems. The popular GCaMP indicators are based on the calcium-binding protein calmodulin and the RS20 peptide. These sensors report neural activity at timescales much slower than electrical signaling, limited by their biophysical properties and trade-offs between sensitivity and speed. We used large-scale screening and structure-guided mutagenesis to develop and optimize several fast and sensitive GCaMP-type indicators. The resulting ‘jGCaMP8’ sensors, based on calmodulin and a fragment of endothelial nitric oxide synthase, have ultra-fast kinetics (rise times, 2 ms) and still feature the highest sensitivity for neural activity reported for any protein-based sensor. jGCaMP8 sensors will allow tracking of larger populations of neurons on timescales relevant to neural computation.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":212833,"datarank":0.6698862177981877,"base_score":4.465908118654584,"endowment":4.465908118654584,"self_citation_contribution":0.6698862177981877,"citation_network_contribution":0.0,"self_endowment_contribution":0.6698862177981877,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":86,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9514,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":684137,"name":"Márton Rózsa","orcid":null,"position":1,"is_corresponding":false},{"id":566037,"name":"Yajie Liang","orcid":"0000-0002-4798-4882","position":2,"is_corresponding":false},{"id":803944,"name":"Daniel Bushey","orcid":"0000-0001-9258-6579","position":3,"is_corresponding":false},{"id":250502,"name":"Ziqiang Wei","orcid":"0000-0002-8694-1260","position":4,"is_corresponding":false},{"id":703116,"name":"Jihong Zheng","orcid":"0000-0002-1247-6087","position":5,"is_corresponding":false},{"id":804736,"name":"Daniel Reep","orcid":null,"position":6,"is_corresponding":false},{"id":714036,"name":"Gerard Joey Broussard","orcid":"0000-0003-1636-2615","position":7,"is_corresponding":false},{"id":803945,"name":"Arthur Tsang","orcid":"0000-0002-2935-8933","position":8,"is_corresponding":false},{"id":804737,"name":"Getahun Tsegaye","orcid":null,"position":9,"is_corresponding":false},{"id":261772,"name":"Sujatha Narayan","orcid":"0000-0002-3741-1319","position":10,"is_corresponding":false},{"id":552516,"name":"Christopher J. Obara","orcid":"0000-0003-1028-6076","position":11,"is_corresponding":false},{"id":803946,"name":"Jing-Xuan Lim","orcid":"0000-0002-5983-9164","position":12,"is_corresponding":false},{"id":557491,"name":"Ronak Patel","orcid":"0000-0002-6945-6466","position":13,"is_corresponding":false},{"id":803947,"name":"Rongwei Zhang","orcid":"0000-0002-3359-0065","position":14,"is_corresponding":false},{"id":261783,"name":"Misha B. Ahrens","orcid":"0000-0002-3457-4462","position":15,"is_corresponding":false},{"id":803948,"name":"Glenn Turner","orcid":"0000-0002-5341-2784","position":16,"is_corresponding":false},{"id":566396,"name":"Samuel S.-H. Wang","orcid":"0000-0002-0490-9786","position":17,"is_corresponding":false},{"id":553804,"name":"Wyatt Korff","orcid":"0000-0001-8396-1533","position":18,"is_corresponding":false},{"id":606862,"name":"Eric R. Schreiter","orcid":"0000-0002-2864-7469","position":19,"is_corresponding":false},{"id":250506,"name":"Karel Svoboda","orcid":"0000-0002-6670-7362","position":20,"is_corresponding":false},{"id":803949,"name":"J. Hasseman","orcid":"0000-0002-0096-7321","position":21,"is_corresponding":false},{"id":758625,"name":"Ilya Kolb","orcid":"0000-0001-5100-849X","position":22,"is_corresponding":false},{"id":44865,"name":"Loren L. Looger","orcid":"0000-0002-7531-1757","position":23,"is_corresponding":false},{"id":803943,"name":"Yan Zhang","orcid":"0000-0001-8376-3190","position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:52:31.378994Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}