{"doi":"10.1101/2021.11.06.467549","title":"The IPF fibroblastic focus is an active collagen biosynthesis factory embedded in a distinct extracellular matrix","abstract":"Abstract Background The Fibroblastic Focus (FF) is the signature lesion of Idiopathic Pulmonary Fibrosis (IPF) where myofibroblasts accumulate and extracellular matrix (ECM) is produced. However, the molecular composition and function of the FF and surrounding tissue remain undefined. Methods Utilizing laser capture microdissection coupled mass spectrometry (LCM-MS), we interrogated the FF, adjacent mature scar, and adjacent alveoli in 6 IPF specimens plus 6 non-fibrotic alveolar specimens as controls. The data were subject to qualitative and quantitative analysis, and validation by immunohistochemistry. Results We found that the protein signature of IPF alveoli is defined by immune deregulation as the strongest category. The IPF mature scar was classified as end-stage fibrosis whereas the FF contained an overabundance of a distinctive ECM compared to non-fibrotic control. Conclusion Spatial proteomics demonstrated distinct protein compositions in the histologically defined regions of IPF tissue. These data revealed that the FF is the main site of collagen biosynthesis and that the alveoli adjacent to the FF are abnormal. This new and essential information will inform future mechanistic studies on mechanisms of IPF progression.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":217998,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9305,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":815364,"name":"Lewis Dingle","orcid":"0000-0002-1886-921X","position":1,"is_corresponding":false},{"id":815365,"name":"M. Angeles Montero","orcid":"0000-0002-9454-2091","position":2,"is_corresponding":false},{"id":815366,"name":"Rajesh Shah","orcid":"0000-0001-8543-0453","position":3,"is_corresponding":false},{"id":815822,"name":"Rajamiyer Venkateswaran","orcid":null,"position":4,"is_corresponding":false},{"id":574419,"name":"John Blaikley","orcid":"0000-0001-7651-5682","position":5,"is_corresponding":false},{"id":810432,"name":"Craig Lawless","orcid":"0000-0002-8240-3430","position":6,"is_corresponding":false},{"id":270346,"name":"Martin A. Schwartz","orcid":"0000-0002-2071-1243","position":7,"is_corresponding":false},{"id":510291,"name":"Jeremy Herrera","orcid":"0000-0003-4845-8494","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:53:24.683898Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}