{"doi":"10.1101/2021.11.05.467477","title":"Age-induced P-bodies become detrimental and shorten the lifespan of yeast","abstract":"Aging is an irreversible process characterized by a progressive loss of homeostasis in cells, which often manifests as protein aggregates. Recently, it has been speculated that aggregates of RNA-binding proteins (RBPs) may go through pathological transitions during aging and drive the progression of ageassociated neurodegenerative diseases. Using Saccharomyces cerevisiae as a model system of aging, we find that P-bodies —an RBP granule that is formed and can be beneficial for cell growth during stress conditions — naturally form during aging without any external stresses and an increase in P-body intensity is negatively correlated with the future lifespan of yeast cells. When mother cells transfer age-induced P-bodies to daughter cells, the mother cells extend lifespan, while the daughter cells grow poorly, suggesting that these age-induced P-bodies may be directly pathological. Furthermore, we find that suppressing acidification of the cytosol during aging slows down the increase in the intensity of P-body foci and extends lifespan. Our data suggest that acidification of the cytosol may facilitate the pathological transition of RBP granules during aging.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":216923,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9552,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":813334,"name":"Shuhao Wang","orcid":"0000-0002-6019-1967","position":1,"is_corresponding":false},{"id":430899,"name":"Yang Li","orcid":"0000-0002-7134-5685","position":2,"is_corresponding":false},{"id":494015,"name":"Nan Hao","orcid":"0000-0003-2857-4789","position":3,"is_corresponding":false},{"id":494014,"name":"Brian M. Zid","orcid":"0000-0003-1876-2479","position":4,"is_corresponding":false},{"id":813333,"name":"Joonhyuk Choi","orcid":"0000-0001-7677-5978","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:53:15.868165Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}