{"doi":"10.1101/2021.10.27.465992","title":"NHR-23 activity is necessary for <i>C. elegans</i> developmental progression and apical extracellular matrix structure and function","abstract":"SUMMARY STATEMENT This work shows how a C. elegans transcription factor controls remodeling of the apical extracellular matrix during development and in which tissues it acts. ABSTRACT Nematode molting is a remarkable process where animals must repeatedly build a new apical extracellular matrix (aECM) beneath a previously built aECM that is subsequently shed. The nuclear hormone receptor NHR-23/NR1F1 is an important regulator of C. elegans molting. NHR-23 expression oscillates in the epidermal epithelium, and soma-specific NHR-23 depletion causes severe developmental delay and death. Tissue-specific RNAi suggests that nhr-23 acts primarily in seam and hypodermal cells. NHR-23 coordinates the expression of factors involved in molting, lipid transport/metabolism, and remodeling of the aECM. NHR-23 depletion causes dampened expression of a nas-37 promoter reporter and a loss of reporter oscillation. The cuticle collagen ROL-6 and zona pellucida protein NOAH-1 display aberrant annular localization and severe disorganization over the seam cells following NHR-23 depletion, while the expression of the adult-specific cuticle collagen BLI-1 is diminished and frequently found in patches. Consistent with these localization defects, the cuticle barrier is severely compromised when NHR-23 is depleted. Together, this work provides insight into how NHR-23 acts in the seam and hypodermal cells to coordinate aECM regeneration during development.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":215236,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9614,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":809934,"name":"An A. Vo","orcid":"0000-0002-3130-6235","position":1,"is_corresponding":false},{"id":337406,"name":"John Clancy","orcid":"0000-0003-2867-9740","position":2,"is_corresponding":false},{"id":809935,"name":"Krista M Myles","orcid":"0000-0002-1281-7793","position":3,"is_corresponding":false},{"id":799900,"name":"Murugesan Pooranachithra","orcid":"0000-0003-3506-7785","position":4,"is_corresponding":false},{"id":809936,"name":"Joseph Aguilera","orcid":"0000-0003-1824-361X","position":5,"is_corresponding":false},{"id":559267,"name":"Max T. Levenson","orcid":"0000-0002-1470-3968","position":6,"is_corresponding":false},{"id":809937,"name":"Chloe Wohlenberg","orcid":"0000-0002-2848-4372","position":7,"is_corresponding":false},{"id":23371,"name":"Andreas Rechtsteiner","orcid":"0000-0001-6031-1087","position":8,"is_corresponding":false},{"id":492342,"name":"James Matthew Ragle","orcid":"0000-0002-6626-2615","position":9,"is_corresponding":false},{"id":281679,"name":"Andrew Chisholm","orcid":"0000-0001-5091-0537","position":10,"is_corresponding":false},{"id":492350,"name":"Jordan D. Ward","orcid":"0000-0001-9870-8936","position":11,"is_corresponding":false},{"id":492348,"name":"Londen C. Johnson","orcid":"0000-0002-8196-4957","position":0,"is_corresponding":true}],"reference_count":140,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:52:55.971300Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}