{"doi":"10.1101/2021.10.26.465695","title":"Centrioles generate a local pulse of Polo/PLK1 activity to initiate mitotic centrosome assembly","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Mitotic centrosomes are formed when centrioles start to recruit large amounts of pericentriolar material (PCM) around themselves in preparation for mitosis. This centrosome “maturation” requires the centrioles and also Polo/PLK1 protein kinase. The PCM comprises several hundred proteins and, in\n                  <jats:italic>Drosophila</jats:italic>\n                  , Polo cooperates with the conserved centrosome proteins Spd-2/CEP192 and Cnn/CDK5RAP2 to assemble a PCM scaffold around the mother centriole that then recruits other PCM client proteins. We show here that in\n                  <jats:italic>Drosophila</jats:italic>\n                  syncytial blastoderm embryos, centrosomal Polo levels rise and fall during the assembly process—peaking, and then starting to decline, even as levels of the PCM scaffold continue to rise and plateau. Experiments and mathematical modelling indicate that a centriolar pulse of Polo activity, potentially generated by the interaction between Polo and its centriole receptor Ana1 (CEP295 in humans), could explain these unexpected scaffold assembly dynamics. We propose that centrioles generate a local pulse of Polo activity prior to mitotic entry to initiate centrosome maturation, explaining why centrioles and Polo/PLK1 are normally essential for this process.\n                </jats:p>","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":null,"id":42103,"datarank":0.3220022944893519,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.010086063237376468,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.010086063237376468,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":4,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":201962,"name":"Zachary M. Wilmott","orcid":"0000-0001-6053-9874","position":1,"is_corresponding":false},{"id":201963,"name":"Saroj Saurya","orcid":"0000-0003-4057-0123","position":2,"is_corresponding":false},{"id":201964,"name":"Ines Alvarez-Rodrigo","orcid":"0000-0003-2181-5535","position":3,"is_corresponding":false},{"id":201965,"name":"Felix Y. Zhou","orcid":"0000-0003-4463-1165","position":4,"is_corresponding":false},{"id":201966,"name":"Kwai-Yin Chau","orcid":null,"position":5,"is_corresponding":false},{"id":201967,"name":"Alain Goriely","orcid":"0000-0002-6436-8483","position":6,"is_corresponding":false},{"id":201968,"name":"Jordan W. Raff","orcid":"0000-0002-4689-1297","position":7,"is_corresponding":false},{"id":201961,"name":"Siu-Shing Wong","orcid":"0000-0002-5327-8466","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18998881","pmcid":null,"openalex_id":"https://openalex.org/W3210475611","authors":[],"funders":[{"funder_name":"UK Research and Innovation","grant_id":"EP/R020205/1","title":"Multiscale modelling and mathematical methods for brain development, trauma, and diseases"},{"funder_name":"Wellcome Trust","grant_id":"215523","title":"Centriole and centrosome assembly"},{"funder_name":"Wellcome Trust","grant_id":"107457","title":"Micron Oxford: super-resolution imaging of cellular dynamics."}],"total_grants":3,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2022,"count":2},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://doi.org/10.1101/2021.10.26.465695","host_type":"repository"},{"url":"https://doi.org/10.1101/2021.10.26.465695","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2021.10.26.465695","host_type":"publisher"},{"url":"https://ora.ox.ac.uk/objects/uuid:bcc5a41f-93a6-4ad5-b33a-3c0543d91901","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2022/02/09/2021.10.26.465695.full.pdf","host_type":""},{"url":"https://doi.org/10.15252/embj.2022110891","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/35505659","host_type":""},{"url":"http://dx.doi.org/10.15252/embj.2022110891","host_type":""},{"url":"https://dx.doi.org/10.1101/2021.10.26.465695","host_type":""}],"fields_of_study":["Microtubule and mitosis dynamics","Genetic and Kidney Cyst Diseases","Photosynthetic Processes and Mechanisms","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Centrosome","Centriole","PLK1","Cell biology","Centrosome cycle","Mitosis","Polo-like kinase","Biology","Cell cycle","Genetics","Cytoskeletal Proteins","Drosophila melanogaster","Animals","Drosophila Proteins","Cell Cycle Proteins","Articles","Protein Serine-Threonine Kinases","Centrioles"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-13T20:09:46.440834Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}