{"doi":"10.1101/2021.10.20.465232","title":"A Recombinant Multivalent Vaccine (rCpa1) Induces Protection for C57BL/6 and HLA Transgenic Mice Against Pulmonary Infection with Both Species of Coccidioides","abstract":"Abstract Coccidioidomycosis is caused by Coccidioides posadasii ( Cp ) and Coccidioides immitis ( Ci ) that have 4-5% differences in their genomic sequences. There is an urgent need to develop a human vaccine against both species. A previously created recombinant antigen (rCpa1) that contains multiple peptides derived from Cp isolate C735 is protective against the autologous isolate. The focus of this study is to evaluate cross-protective efficacy and immune correlates by the rCpa1- based vaccine against both species of Coccidioides . DNA sequence analyses of the homologous genes for the rCpa1 antigen were conducted for 39 and 17 clinical isolates of Cp and Ci , respectively. Protective efficacy and vaccine-induced immunity were evaluated for both C57BL/6 and human HLA-DR4 transgenic mice against 5 highly virulent isolates of Cp and Ci . There are a total of 7 amino acid substitutions in the rCpa1 antigen between Cp and Ci . Both C57BL/6 and HLA-DR4 mice that were vaccinated with a rCpa1 vaccine resulted in significant reduction of fungal burden and increased numbers of IFN-γ- and IL-17-producing CD4 + T cells in the first 2 weeks post-challenge. These data support that rCpa1 has cross-protection activity against Cp and Ci pulmonary infection through activation of early Th1 and Th17 responses.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":219491,"datarank":0.18763657232091507,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.02284472902069863,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.02284472902069863,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9468,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":818127,"name":"Komali Devi Pentakota","orcid":"0009-0002-4303-6209","position":1,"is_corresponding":false},{"id":818731,"name":"Yu-Rou Liao","orcid":null,"position":2,"is_corresponding":false},{"id":256558,"name":"Hao Zhang","orcid":"0000-0002-4582-2556","position":3,"is_corresponding":false},{"id":276828,"name":"Nathan P. Wiederhold","orcid":"0000-0002-2225-5122","position":4,"is_corresponding":false},{"id":361133,"name":"Gary R. Ostroff","orcid":"0000-0003-4202-4556","position":5,"is_corresponding":false},{"id":320386,"name":"Chiung-Yu Hung","orcid":"0000-0003-1091-3420","position":6,"is_corresponding":false},{"id":818126,"name":"Althea Campuzano","orcid":"0000-0003-3159-7113","position":0,"is_corresponding":true}],"reference_count":46,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:53:38.409982Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}