{"doi":"10.1101/2021.10.20.465140","title":"Dynamic chromosomal interactions and control of heterochromatin positioning by Ki-67","abstract":"Abstract Ki-67 is a chromatin-associated protein with a dynamic distribution pattern throughout the cell cycle, and is thought to be involved in chromatin organization. Lack of genomic interaction maps has hampered a detailed understanding of its roles, particularly during interphase. By pA-DamID mapping in human cell lines we found that Ki-67 associates with large genomic domains that overlap mostly with late-replicating regions. Early in interphase, when Ki-67 is present in pre-nucleolar bodies, it interacts with these domains on all chromosomes. However, later in interphase, when Ki-67 is confined to nucleoli, it shows a striking shift towards small chromosomes. Nucleolar perturbations indicate that these cell cycle dynamics correspond to nucleolar maturation during interphase, and suggest that nucleolar sequestration of Ki-67 limits its interactions with larger chromosomes. Furthermore, we demonstrate that Ki-67 does not detectably control chromatin-chromatin interactions during interphase, but it competes with the nuclear lamina for interaction with late-replicating DNA, and it controls replication timing of (peri)centromeric regions. Together, these results reveal a highly dynamic choreography of genome interactions and roles for Ki-67 in heterochromatin organization.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":217944,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":622911,"name":"Stefano Giustino Manzo","orcid":"0000-0002-6911-3527","position":1,"is_corresponding":false},{"id":804753,"name":"Athanasios E Vouzas","orcid":null,"position":2,"is_corresponding":false},{"id":808197,"name":"Ning Qing Liu","orcid":"0000-0002-3151-638X","position":3,"is_corresponding":false},{"id":619876,"name":"Hans Teunissen","orcid":null,"position":4,"is_corresponding":false},{"id":53936,"name":"Elzo de Wit","orcid":"0000-0003-2883-1415","position":5,"is_corresponding":false},{"id":42992,"name":"David M. Gilbert","orcid":"0000-0001-8087-9737","position":6,"is_corresponding":false},{"id":39702,"name":"Bas van Steensel","orcid":"0000-0002-0284-0404","position":7,"is_corresponding":false},{"id":261862,"name":"Tom van Schaik","orcid":"0000-0001-7850-5074","position":0,"is_corresponding":true}],"reference_count":77,"raw_metadata":null,"created_at":"2026-07-18T23:53:24.683898Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}