{"doi":"10.1101/2021.10.13.464307","title":"A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses","abstract":"The repeated emergence of highly pathogenic human coronaviruses as well as their evolving variants highlight the need to develop potent and broad-spectrum antiviral therapeutics and vaccines. By screening monoclonal antibodies (mAbs) isolated from COVID-19-convalescent patients, we found one mAb, 2-36, with cross-neutralizing activity against SARS-CoV. We solved the cryo-EM structure of 2-36 in complex with SARS-CoV-2 or SARS-CoV spike, revealing a highly conserved epitope in the receptor-binding domain (RBD). Antibody 2-36 neutralized not only all current circulating SARS-CoV-2 variants and SARS-COV, but also a panel of bat and pangolin sarbecoviruses that can use human angiotensin-converting enzyme 2 (ACE2) as a receptor. We selected 2-36-escape viruses in vitro and confirmed that K378T in SARS-CoV-2 RBD led to viral resistance. Taken together, 2-36 represents a strategic reserve drug candidate for the prevention and treatment of possible diseases caused by pre-emergent SARS-related coronaviruses. Its epitope defines a promising target for the development of a pan-sarbecovirus vaccine.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":214434,"datarank":0.3596842909197557,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.0,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9508,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":615367,"name":"Ryan G. Casner","orcid":"0000-0002-2910-0908","position":1,"is_corresponding":false},{"id":105620,"name":"Manoj S. Nair","orcid":"0000-0002-5994-3957","position":2,"is_corresponding":false},{"id":105621,"name":"Jian Yu","orcid":"0000-0001-8604-4538","position":3,"is_corresponding":false},{"id":482831,"name":"Yicheng Guo","orcid":"0000-0001-5357-660X","position":4,"is_corresponding":false},{"id":616934,"name":"Maple Wang","orcid":null,"position":5,"is_corresponding":false},{"id":78590,"name":"Jasper Fuk‐Woo Chan","orcid":"0000-0001-6336-6657","position":6,"is_corresponding":false},{"id":105628,"name":"Gabriele Cerutti","orcid":"0000-0002-9081-7561","position":7,"is_corresponding":false},{"id":350800,"name":"Sho Iketani","orcid":"0000-0002-3733-9556","position":8,"is_corresponding":false},{"id":105618,"name":"Lihong Liu","orcid":"0000-0003-3128-8513","position":9,"is_corresponding":false},{"id":105636,"name":"Zizhang Sheng","orcid":"0000-0002-3253-3309","position":10,"is_corresponding":false},{"id":105632,"name":"Zhiwei Chen","orcid":"0000-0002-4511-2888","position":11,"is_corresponding":false},{"id":72027,"name":"Kwok‐Yung Yuen","orcid":"0000-0002-2083-1552","position":12,"is_corresponding":false},{"id":105633,"name":"Peter D. Kwong","orcid":"0000-0003-3560-232X","position":13,"is_corresponding":false},{"id":105637,"name":"Yaoxing Huang","orcid":"0000-0001-6270-1644","position":14,"is_corresponding":false},{"id":105638,"name":"Lawrence Shapiro","orcid":"0000-0001-9943-8819","position":15,"is_corresponding":false},{"id":104013,"name":"David D. Ho","orcid":"0000-0003-1627-149X","position":16,"is_corresponding":false},{"id":105619,"name":"Pengfei Wang","orcid":"0000-0003-2454-7652","position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:52:46.604690Z","pmid":"34671769","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}