{"doi":"10.1101/2021.09.17.460763","title":"MinION nanopore sequencing provides similar methylation estimates to Sanger bisulfite sequencing in the\n                  <i>TRPA1</i>\n                  promoter region","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Bisulfite sequencing has long been considered the gold standard for measurement of DNA methylation at single CpG resolution. In the meantime, several new approaches have been developed, which are regarded as less error-prone. Since these errors were shown to be sequence-specific, we aimed to verify the methylation data of a particular region of the\n                  <jats:italic>TRPA1</jats:italic>\n                  promoter obtained from our previous studies. For this purpose, we compared methylation rates obtained via direct bisulfite sequencing and nanopore sequencing. Thus, we were able to confirm our previous findings to a large extent.\n                </jats:p>","journal":null,"year":null,"id":594738,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1522630,"name":"Kirsten Jahn","orcid":null,"position":1,"is_corresponding":false},{"id":1522631,"name":"Hansi Pathak","orcid":null,"position":2,"is_corresponding":false},{"id":1522632,"name":"Alexandra Burkert","orcid":null,"position":3,"is_corresponding":false},{"id":842795,"name":"Gunnar Schmidt","orcid":null,"position":4,"is_corresponding":false},{"id":1522633,"name":"Lutz Wiehlmann","orcid":null,"position":5,"is_corresponding":false},{"id":1522634,"name":"Colin Davenport","orcid":null,"position":6,"is_corresponding":false},{"id":271078,"name":"Björn Brändl","orcid":"0000-0003-1044-1355","position":7,"is_corresponding":false},{"id":851366,"name":"Frank Müller","orcid":"0000-0001-9478-8430","position":8,"is_corresponding":false},{"id":1213418,"name":"Andreas Leffler","orcid":"0000-0003-1121-6387","position":9,"is_corresponding":false},{"id":1522635,"name":"Maximilian Deest","orcid":null,"position":10,"is_corresponding":false},{"id":1522637,"name":"Helge Frieling","orcid":null,"position":11,"is_corresponding":false},{"id":1522629,"name":"Sara Gombert","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"MinION nanopore sequencing provides similar methylation estimates to Sanger bisulfite sequencing in the\n                  <i>TRPA1</i>\n                  promoter region","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Bisulfite sequencing has long been considered the gold standard for measurement of DNA methylation at single CpG resolution. In the meantime, several new approaches have been developed, which are regarded as less error-prone. Since these errors were shown to be sequence-specific, we aimed to verify the methylation data of a particular region of the\n                  <jats:italic>TRPA1</jats:italic>\n                  promoter obtained from our previous studies. For this purpose, we compared methylation rates obtained via direct bisulfite sequencing and nanopore sequencing. Thus, we were able to confirm our previous findings to a large extent.\n                </jats:p>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23304386","pmcid":null,"openalex_id":"https://openalex.org/W3200718802","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2021/09/18/2021.09.17.460763.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2021/09/18/2021.09.17.460763.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2021.09.17.460763","host_type":"publisher"},{"url":"https://doi.org/10.1101/2021.09.17.460763","host_type":"repository"}],"fields_of_study":["Electrocatalysts for Energy Conversion","MicroRNA in disease regulation","CRISPR and Genetic Engineering"],"mesh_terms":[],"keywords":["Nanopore sequencing","Bisulfite sequencing","Bisulfite","Sanger sequencing","Minion","Methylated DNA immunoprecipitation","Illumina Methylation Assay","Methylation","DNA methylation","Computational biology","CpG site","DNA sequencing","Biology","Genetics","Nanopore","Gene","Gene expression"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T15:26:28.116374Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}