{"doi":"10.1101/2021.09.15.460487","title":"Synthetic Multiantigen MVA Vaccine COH04S1 Protects Against SARS-CoV-2 in Syrian Hamsters and Non-Human Primates","abstract":"Second-generation COVID-19 vaccines could contribute to establish protective immunity against SARS-CoV-2 and its emerging variants. We developed COH04S1, a synthetic multiantigen Modified Vaccinia Ankara-based SARS-CoV-2 vaccine that co-expresses spike and nucleocapsid antigens. Here, we report COH04S1 vaccine efficacy in animal models. We demonstrate that intramuscular or intranasal vaccination of Syrian hamsters with COH04S1 induces robust Th1-biased antigen-specific humoral immunity and cross-neutralizing antibodies (NAb) and protects against weight loss, lower respiratory tract infection, and lung injury following intranasal SARS-CoV-2 challenge. Moreover, we demonstrate that single-dose or two-dose vaccination of non-human primates with COH04S1 induces robust antigen-specific binding antibodies, NAb, and Th1-biased T cells, protects against both upper and lower respiratory tract infection following intranasal/intratracheal SARS-CoV-2 challenge, and triggers potent post-challenge anamnestic antiviral responses. These results demonstrate COH04S1-mediated vaccine protection in animal models through different vaccination routes and dose regimens, complementing ongoing investigation of this multiantigen SARS-CoV-2 vaccine in clinical trials.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":215571,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9521,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":279373,"name":"Vu H. Nguyen","orcid":"0000-0003-3974-7040","position":1,"is_corresponding":false},{"id":280345,"name":"Yoonsuh Park","orcid":null,"position":2,"is_corresponding":false},{"id":279372,"name":"Heidi Contreras","orcid":"0000-0003-4476-9156","position":3,"is_corresponding":false},{"id":810359,"name":"Veronica Karpinski","orcid":"0000-0002-4562-0583","position":4,"is_corresponding":false},{"id":346159,"name":"Katelyn Faircloth","orcid":null,"position":5,"is_corresponding":false},{"id":274825,"name":"Jenny Nguyen","orcid":"0000-0003-2952-6877","position":6,"is_corresponding":false},{"id":276476,"name":"Mindy Kha","orcid":null,"position":7,"is_corresponding":false},{"id":810901,"name":"Daisy Johnson","orcid":null,"position":8,"is_corresponding":false},{"id":280344,"name":"Joy Martinez","orcid":null,"position":9,"is_corresponding":false},{"id":280346,"name":"Angelina Iniguez","orcid":null,"position":10,"is_corresponding":false},{"id":279374,"name":"Qiao Zhou","orcid":"0000-0003-2666-2283","position":11,"is_corresponding":false},{"id":280347,"name":"Teodora Kaltcheva","orcid":null,"position":12,"is_corresponding":false},{"id":380347,"name":"Paul Frankel","orcid":"0000-0003-2231-6121","position":13,"is_corresponding":false},{"id":618561,"name":"Swagata Kar","orcid":"0000-0003-3702-6207","position":14,"is_corresponding":false},{"id":760801,"name":"Ankur Sharma","orcid":"0000-0002-6862-136X","position":15,"is_corresponding":false},{"id":106758,"name":"Hanné Andersen","orcid":null,"position":16,"is_corresponding":false},{"id":4374,"name":"Mark G. Lewis","orcid":"0000-0001-7852-0135","position":17,"is_corresponding":false},{"id":279379,"name":"Yuriy Shostak","orcid":"0000-0003-2891-7905","position":18,"is_corresponding":false},{"id":274828,"name":"Felix Wussow","orcid":"0000-0001-7624-6734","position":19,"is_corresponding":false},{"id":274827,"name":"Don J. Diamond","orcid":"0000-0003-3579-7083","position":20,"is_corresponding":false},{"id":274822,"name":"Flavia Chiuppesi","orcid":"0000-0002-2921-5377","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-18T23:52:55.971300Z","pmid":"34545366","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}