{"doi":"10.1101/2021.09.10.459857","title":"Effectors from a Bacterial Vector-Borne Pathogen Exhibit Diverse Subcellular Localization, Expression Profiles and Manipulation of Plant Defense","abstract":"SUMMARY Climate change is predicted to increase the prevalence of vector borne disease due to expansion of insect populations. Candidatus Liberibacter solanacearum ( C Lso) is a phloem-limited pathogen associated with multiple economically important diseases in Solanaceous crops. Little is known about the strategies and pathogenicity factors C Lso uses to colonize vector and host. We determined the C Lso effector repertoire by predicting SEC secreted proteins across four different C Lso haplotypes, investigated effector localization in planta , and profiled effector expression in vector and host. The localization of C Lso effectors in Nicotiana revealed diverse eukaryotic subcellular targets. The majority of tested effectors were unable to suppress plant immune responses, indicating they possess unique activities. Expression profiling in tomato and the psyllid Bactericera cockerelli indicated C Lso differentially interacts with its host and vector and can switch effector expression in response to the environment. This study reveals C Lso effectors possess complex expression patterns, target diverse host organelles and the majority are unable to suppress host immune responses. A mechanistic understanding of Lso effector function will reveal novel targets and provide insight into phloem biology.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":219310,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9587,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":564450,"name":"Jie Zhu","orcid":"0000-0002-5217-6249","position":1,"is_corresponding":false},{"id":58072,"name":"Andrew Breakspear","orcid":"0000-0002-1863-3012","position":2,"is_corresponding":false},{"id":590294,"name":"Shree Prasad Thapa","orcid":"0000-0001-6524-6261","position":3,"is_corresponding":false},{"id":233502,"name":"Tania Y. Toruño","orcid":"0000-0002-9220-016X","position":4,"is_corresponding":false},{"id":817878,"name":"Laura M. Perilla‐Henao","orcid":"0000-0001-9473-4985","position":5,"is_corresponding":false},{"id":778169,"name":"Clare L. Casteel","orcid":"0000-0003-3678-0187","position":6,"is_corresponding":false},{"id":657472,"name":"Christine Faulkner","orcid":"0000-0003-3905-8077","position":7,"is_corresponding":false},{"id":233503,"name":"Gitta Coaker","orcid":"0000-0003-0899-2449","position":8,"is_corresponding":false},{"id":817877,"name":"Paola A. Reyes Caldas","orcid":"0000-0002-6838-7585","position":0,"is_corresponding":true}],"reference_count":92,"raw_metadata":null,"created_at":"2026-07-18T23:53:38.409982Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}