{"doi":"10.1101/2021.09.10.459800","title":"pH-dependent polymorphism of the structure of SARS-CoV-2 nsp7","abstract":"Abstract The solution structure of SARS-CoV-2 nonstructural protein 7 (nsp7) at pH 7.0 has been determined by NMR spectroscopy. nsp7 is conserved in the coronavirinae subfamily and is an essential co-factor of the viral RNA-dependent RNA polymerase for active and processive replication. Similar to the previously deposited structures of SARS-CoV-1 nsp7 at acidic and basic conditions, SARS-CoV-2 nsp7 has a helical bundle folding at neutral pH. Remarkably, the α4 helix shows gradual dislocation from the core α2-α3 structure as pH increases from 6.5 to 7.5. The protonation state of residue H36 contributes to the change of nsp7’s intramolecular interactions, and thus, to the structural variation near-neutral pH. Spin-relaxation results revealed that all three loop regions in nsp7 possess dynamic properties associated with this structural variation.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":217838,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9405,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":346055,"name":"Marco Tonelli","orcid":"0000-0002-7700-5745","position":1,"is_corresponding":false},{"id":815130,"name":"Mehdi Rahimi","orcid":"0000-0001-7812-7333","position":2,"is_corresponding":false},{"id":526697,"name":"Thomas K. Anderson","orcid":"0000-0002-8076-3985","position":3,"is_corresponding":false},{"id":558658,"name":"Robert N. Kirchdoerfer","orcid":"0000-0002-5974-2709","position":4,"is_corresponding":false},{"id":526698,"name":"Katherine A. Henzler‐Wildman","orcid":"0000-0002-5295-2121","position":5,"is_corresponding":false},{"id":410846,"name":"Woonghee Lee","orcid":"0000-0002-0964-203X","position":6,"is_corresponding":false},{"id":815129,"name":"Yeongjoon Lee","orcid":"0000-0002-9156-6477","position":0,"is_corresponding":true}],"reference_count":42,"raw_metadata":null,"created_at":"2026-07-18T23:53:24.683898Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}