{"doi":"10.1101/2021.09.08.459553","title":"Characterization of apicomplexan amino acid transporters (ApiATs) in the malaria parasite <i>Plasmodium falciparum</i>","abstract":"ABSTRACT During the symptomatic human blood phase, malaria parasites replicate within red blood cells. Parasite proliferation relies on the uptake of nutrients, such as amino acids, from the host cell and the blood plasma, requiring transport across multiple membranes. Amino acids are delivered to the parasite through the parasite surrounding vacuolar compartment by specialized nutrient-permeable channels of the erythrocyte membrane and the parasitophorous vacuole membrane (PVM). However, further transport of amino acid across the parasite plasma membrane (PPM) is currently not well characterized. In this study, we focused on a family of Apicomplexan amino acid transporters (ApiATs) that comprises five members in Plasmodium falciparum . First, we localized four of the Pf ApiATs at the PPM using endogenous GFP-tagging. Next, we applied reverse genetic approaches to probe into their essentiality during asexual replication and gametocytogenesis. Upon inducible knockdown and targeted gene disruption a reduced asexual parasite proliferation was detected for Pf ApiAT2 and Pf ApiAT4. Functional inactivation of individual Pf ApiATs targeted in this study had no effect on gametocyte development. Our data suggest that individual Pf ApiATs are partially redundant during asexual in vitro proliferation and fully redundant during gametocytogenesis of P. falciparum parasites. IMPORTANCE Malaria parasites live and multiply inside cells. To facilitate their extremely fast intracellular proliferation they hijack and transform their host cells. This also requires the active uptake of nutrients, such as amino acids, from the host cell and the surrounding environment through various membranes that are the consequence of the parasite’s intracellular lifestyle. In this manuscript we focus on a family of putative amino acid transporters termed ApiAT. We show expression and localization of four transporters in the parasite plasma membrane of Plasmodium falciparum -infected erythrocytes that represent one interface of the pathogen to its host cell. We probed into the impact of functional inactivation of individual transporters on parasite growth in asexual and sexual blood stages of P. falciparum and reveal that only two of them show a modest but significant reduction in parasite proliferation but no impact on gametocytogenesis pointing towards redundancy within this transporter family.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":226713,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9618,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":725549,"name":"Carolina van Gelder","orcid":"0000-0002-8109-3098","position":1,"is_corresponding":false},{"id":725550,"name":"Gwendolin Fuchs","orcid":"0000-0001-9294-6984","position":2,"is_corresponding":false},{"id":725551,"name":"Julia Mareike Ruge","orcid":"0000-0002-6295-0293","position":3,"is_corresponding":false},{"id":725552,"name":"Emma Pietsch","orcid":"0000-0003-4744-1666","position":4,"is_corresponding":false},{"id":707276,"name":"Josie L. Ferreira","orcid":"0000-0002-4411-6131","position":5,"is_corresponding":false},{"id":725553,"name":"Soraya Safavi","orcid":"0000-0001-9989-0952","position":6,"is_corresponding":false},{"id":687099,"name":"Heidrun von Thien","orcid":null,"position":7,"is_corresponding":false},{"id":725554,"name":"Paul-Christian Burda","orcid":"0000-0003-0461-4352","position":8,"is_corresponding":false},{"id":686091,"name":"Paolo Mesén-Ramírez","orcid":"0000-0001-7842-5867","position":9,"is_corresponding":false},{"id":457028,"name":"Tobias Spielmann","orcid":"0000-0002-3968-4601","position":10,"is_corresponding":false},{"id":725555,"name":"Jan Strauss","orcid":"0000-0002-6208-791X","position":11,"is_corresponding":false},{"id":457027,"name":"Tim‐Wolf Gilberger","orcid":"0000-0002-7965-8272","position":12,"is_corresponding":false},{"id":686097,"name":"Anna Bachmann","orcid":"0000-0001-8397-7308","position":13,"is_corresponding":false},{"id":725548,"name":"Jan Stephan Wichers","orcid":"0000-0002-0599-1742","position":0,"is_corresponding":true}],"reference_count":87,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:54:38.004707Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}