{"doi":"10.1101/2021.09.06.459143","title":"Novel effector recognition capacity engineered into a paired NLR complex","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  The Arabidopsis\n                  <jats:italic>RRS1-R Resistance</jats:italic>\n                  gene confers recognition of the bacterial acetyltransferase PopP2 and another bacterial effector, AvrRps4. The\n                  <jats:italic>RRS1-S</jats:italic>\n                  allele recognizes AvrRps4 but not PopP2.\n                  <jats:italic>RRS1- R/RRS1-S</jats:italic>\n                  heterozygotes cannot recognize PopP2.\n                  <jats:italic>RRS1-R</jats:italic>\n                  and\n                  <jats:italic>RRS1-S</jats:italic>\n                  also suppress the constitutive RPS4-dependent autoactivity of\n                  <jats:italic>RRS1-R</jats:italic>\n                  <jats:sup>\n                    <jats:italic>slh1</jats:italic>\n                  </jats:sup>\n                  . Phytoplasmas cause important plant diseases, and their effectors can cause degradation of specific host proteins. We tested whether attaching a pathogen effector-dependent degron to RRS1-R, enabling its degradation by phytoplasma effector SAP05, could derepress RRS1-R\n                  <jats:sup>\n                    <jats:italic>slh1</jats:italic>\n                  </jats:sup>\n                  autoactivity, resulting in SAP05-dependent resistance. In transient assays in tobacco, RRS1-R-derived constructs can confer a hypersensitive response (HR) to SAP05. However, phytoplasma infection assays in transgenic Arabidopsis resulted in delayed disease symptoms but not full resistance. We provide a proof-of-concept strategy utilizing the recessiveness of a plant immune receptor gene to engineer recognition of a pathogen effector that promotes degradation of a specific host protein.\n                </jats:p>","journal":null,"year":null,"id":592195,"datarank":0.9979526971563255,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.591745166990994,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.591745166990994,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":13,"citers_with_citation_signal":13,"citers_with_endowment":13,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1166186,"name":"Weijie Huang","orcid":"0000-0002-2481-1188","position":1,"is_corresponding":false},{"id":1515367,"name":"Zane Duxbury","orcid":"0000-0001-8623-0525","position":2,"is_corresponding":false},{"id":1338721,"name":"Saskia A. Hogenhout","orcid":"0000-0003-1371-5606","position":3,"is_corresponding":false},{"id":18798,"name":"Jonathan D. G. Jones","orcid":"0000-0002-4953-261X","position":4,"is_corresponding":false},{"id":140857,"name":"Shanshan Wang","orcid":"0000-0003-3881-0087","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Novel effector recognition capacity engineered into a paired NLR complex","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  The Arabidopsis\n                  <jats:italic>RRS1-R Resistance</jats:italic>\n                  gene confers recognition of the bacterial acetyltransferase PopP2 and another bacterial effector, AvrRps4. The\n                  <jats:italic>RRS1-S</jats:italic>\n                  allele recognizes AvrRps4 but not PopP2.\n                  <jats:italic>RRS1- R/RRS1-S</jats:italic>\n                  heterozygotes cannot recognize PopP2.\n                  <jats:italic>RRS1-R</jats:italic>\n                  and\n                  <jats:italic>RRS1-S</jats:italic>\n                  also suppress the constitutive RPS4-dependent autoactivity of\n                  <jats:italic>RRS1-R</jats:italic>\n                  <jats:sup>\n                    <jats:italic>slh1</jats:italic>\n                  </jats:sup>\n                  . Phytoplasmas cause important plant diseases, and their effectors can cause degradation of specific host proteins. We tested whether attaching a pathogen effector-dependent degron to RRS1-R, enabling its degradation by phytoplasma effector SAP05, could derepress RRS1-R\n                  <jats:sup>\n                    <jats:italic>slh1</jats:italic>\n                  </jats:sup>\n                  autoactivity, resulting in SAP05-dependent resistance. In transient assays in tobacco, RRS1-R-derived constructs can confer a hypersensitive response (HR) to SAP05. However, phytoplasma infection assays in transgenic Arabidopsis resulted in delayed disease symptoms but not full resistance. We provide a proof-of-concept strategy utilizing the recessiveness of a plant immune receptor gene to engineer recognition of a pathogen effector that promotes degradation of a specific host protein.\n                </jats:p>","is_dataset_classified":null,"base_score":2.70805020110221,"endowment":2.70805020110221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26657633","pmcid":null,"openalex_id":"https://openalex.org/W3197238340","authors":[],"funders":[{"funder_name":"European Commission","grant_id":"669926","title":"Design and redesign of a plant immune receptor complex"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2021,"count":1},{"year":2022,"count":4},{"year":2023,"count":5},{"year":2024,"count":1},{"year":2025,"count":3}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2021/09/06/2021.09.06.459143.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2021/09/06/2021.09.06.459143.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2021.09.06.459143","host_type":"publisher"},{"url":"https://doi.org/10.1101/2021.09.06.459143","host_type":"repository"},{"url":"https://dx.doi.org/10.1101/2021.09.06.459143","host_type":""}],"fields_of_study":["Plant-Microbe Interactions and Immunity","Plant Pathogenic Bacteria Studies","Phytoplasmas and Hemiptera pathogens","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Effector","Arabidopsis","Biology","Degron","Gene","Phytoplasma","Pathogen","Transgene","Petunia","Plant disease resistance","Cell biology","Genetics","Mutant","Polymerase chain reaction","Ubiquitin"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"},{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-26T12:36:28.339379Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}