{"doi":"10.1101/2021.09.01.458551","title":"Mixed Origins: HIV gp120-specific memory develops from pre-existing memory and naïve B cells following vaccination in humans","abstract":"Abstract The most potent and broad HIV envelope (Env)-specific antibodies often when reverted to their inferred germline versions representing the naïve B cell receptor, fail to bind Env suggesting that the initial responding B cell population is not exclusively comprised of a naïve population, but also a pre-existing cross-reactive antigen-experienced B cell pool that expands following Env exposure. Previously we isolated gp120-reactive monoclonal antibodies (mAbs) from participants in HVTN 105, an HIV vaccine trial. Using deep sequencing VH-lineage tracking we identified several of these mAb lineages in pre-immune peripheral blood. Several of these pre-immune lineages also persisted in the bone marrow, including CD138+ long-lived plasma cell compartment, ∼7 months after the final vaccination. The majority of the pre-immune lineage members included IgM, however IgG and IgA members were also prevalent and exhibited somatic hypermutation. These results suggest that vaccine-induced gp120-specific antibody lineages originate from both naïve and cross-reactive memory B cells.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":221581,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9504,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":432139,"name":"Michael S. Piepenbrink","orcid":"0000-0001-7517-6412","position":1,"is_corresponding":false},{"id":181722,"name":"Christopher Fucile","orcid":null,"position":2,"is_corresponding":false},{"id":443038,"name":"Catherine A. Bunce","orcid":null,"position":3,"is_corresponding":false},{"id":821692,"name":"Li‐Xing Man","orcid":"0000-0003-4981-2096","position":4,"is_corresponding":false},{"id":284141,"name":"Jane L. Liesveld","orcid":"0000-0002-3386-8469","position":5,"is_corresponding":false},{"id":293771,"name":"Alexander F. Rosenberg","orcid":null,"position":6,"is_corresponding":false},{"id":608916,"name":"Michael C. Keefer","orcid":"0000-0001-9048-3621","position":7,"is_corresponding":false},{"id":432142,"name":"James J. Kobie","orcid":"0000-0001-8069-0272","position":8,"is_corresponding":false},{"id":443035,"name":"Madhubanti Basu","orcid":null,"position":0,"is_corresponding":true}],"reference_count":74,"raw_metadata":null,"created_at":"2026-07-18T23:53:55.215284Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}