{"doi":"10.1101/2021.08.24.457384","title":"PRMT5 promotes symmetric dimethylation of RNA processing proteins and modulates activated T cell alternative splicing and Ca <sup>2+</sup> /NFAT signaling","abstract":"Abstract Protein Arginine Methyltransferase (PRMT) 5 is the major type 2 methyltransferase catalyzing symmetric dimethylation (SDM) of arginine. PRMT5 inhibition or deletion in CD4 Th cells reduces TcR engagement-induced IL-2 production and Th cell expansion and confers protection against experimental autoimmune encephalomyelitis (EAE), the animal model of Multiple Sclerosis. However, the mechanisms by which PRMT5 modulates T helper (Th) cell proliferation are still not completely understood and neither are the methylation targets in T cells. In this manuscript, we uncover the role of PRMT5 on alternative splicing (AS) in activated T cells and identify several targets of PRMT5 SDM involved in splicing. In addition, we find a possible link between PRMT5 mediated AS of Trpm4 (Transient Receptor Potential Cation Channel Subfamily M Member 4) and TcR/NFAT signaling/IL-2 production. This understanding may guide development of drugs targeting these processes to benefit patients with T cell-mediated diseases.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":226209,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9585,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":270975,"name":"Kelsi O West","orcid":"0000-0002-9632-7205","position":1,"is_corresponding":false},{"id":754528,"name":"Shridhar Sanghvi","orcid":"0000-0001-9254-7078","position":2,"is_corresponding":false},{"id":302673,"name":"George Laliotis","orcid":"0000-0001-9205-8258","position":3,"is_corresponding":false},{"id":754529,"name":"Laura M. Agosto","orcid":"0000-0003-0271-6574","position":4,"is_corresponding":false},{"id":302511,"name":"Kristen W. Lynch","orcid":"0000-0002-0120-8079","position":5,"is_corresponding":false},{"id":302674,"name":"Philip N. Tsichlis","orcid":"0000-0002-5300-0448","position":6,"is_corresponding":false},{"id":338876,"name":"Harpreet Singh","orcid":"0000-0002-6135-1897","position":7,"is_corresponding":false},{"id":270976,"name":"Kristin L. Patrick","orcid":"0000-0003-2442-4679","position":8,"is_corresponding":false},{"id":232994,"name":"Mireia Guerau‐de‐Arellano","orcid":"0000-0002-0600-4593","position":9,"is_corresponding":false},{"id":302668,"name":"Shouvonik Sengupta","orcid":"0000-0003-1680-1315","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-18T23:54:34.185847Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}