{"doi":"10.1101/2021.07.29.454039","title":"Dual Roles for Nuclear RNAi Argonautes in <i>C. elegans</i> Dosage Compensation","abstract":"ABSTRACT Dosage compensation involves chromosome-wide gene regulatory mechanisms which impact higher order chromatin structure and are crucial for organismal health. Using a genetic approach, we identified Argonaute genes which promote dosage compensation in C. elegans . Dosage compensation in C. elegans hermaphrodites is initiated by the silencing of xol-1 and subsequent activation of the Dosage Compensation Complex (DCC) which binds to both hermaphrodite X chromosomes and reduces transcriptional output by twofold. A hallmark phenotype of dosage compensation mutants is decondensation of the X chromosomes. We characterized this phenotype in Argonaute mutants using X chromosome paint probe and fluorescence microscopy. We found that while nuclear Argonaute mutants hrde-1 and nrde-3 exhibit de-repression of xol-1 transcripts, they also effect X chromosome condensation in a xol-1 -independent manner. We also characterized the physiological contribution of Argonaute genes to dosage compensation using genetic assays and find that hrde-1 and nrde-3 , together with the piRNA Argonaute prg-1 , contribute to healthy dosage compensation both upstream and downstream of xol-1 .","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":225820,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":828568,"name":"Bahaar Chawla","orcid":"0000-0001-5969-7525","position":1,"is_corresponding":false},{"id":828569,"name":"Eshna Jash","orcid":"0000-0003-1858-5126","position":2,"is_corresponding":false},{"id":828570,"name":"Lillian E. Tushman","orcid":"0000-0001-7724-1273","position":3,"is_corresponding":false},{"id":828914,"name":"Rebecca A. Haines","orcid":null,"position":4,"is_corresponding":false},{"id":550258,"name":"Györgyi Csankovszki","orcid":"0000-0001-5918-034X","position":5,"is_corresponding":false},{"id":828567,"name":"Michael B. Davis","orcid":"0000-0003-3416-3038","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-18T23:54:30.292454Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}