{"doi":"10.1101/2021.07.20.452795","title":"Displacement statistics of unhindered single molecules show no enhanced diffusion in enzymatic reactions","abstract":"ABSTRACT Recent studies have sparked heated debate over whether catalytical reactions would enhance the diffusion coefficients D of enzymes. Through high statistics of the transient (600 μs) displacements of unhindered single molecules freely diffusing in common buffers, we here quantify D for four highly contested enzymes under catalytic turnovers. We thus formulate how precisions of better than ±1% may be achieved for D at the 95% confidence level, and show no changes in diffusivity for catalase, urease, aldolase, and alkaline phosphatase under the application of wide concentration ranges of substrates. Our single-molecule approach thus overcomes potential limitations and artifacts underscored by recent studies to show no enhanced diffusion in enzymatic reactions. Table of Contents artwork","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":225795,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9425,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":828546,"name":"Ha H. Park","orcid":"0000-0003-2290-7992","position":1,"is_corresponding":false},{"id":583510,"name":"Kun Chen","orcid":"0000-0001-6139-3627","position":2,"is_corresponding":false},{"id":552200,"name":"Rui Yan","orcid":"0000-0003-3391-133X","position":3,"is_corresponding":false},{"id":632289,"name":"Wan Li","orcid":"0000-0001-5751-3550","position":4,"is_corresponding":false},{"id":218681,"name":"Ke Xu","orcid":"0000-0002-2788-194X","position":5,"is_corresponding":false},{"id":828545,"name":"Alexander A. Choi","orcid":"0000-0003-2119-8995","position":0,"is_corresponding":true}],"reference_count":38,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:54:30.292454Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}