{"doi":"10.1101/2021.07.18.452838","title":"TAF4b transcription networks regulating early oocyte differentiation","abstract":"ABSTRACT Establishment of a healthy ovarian reserve is contingent upon numerous regulatory pathways during embryogenesis. Previously, mice lacking TBP-associated factor 4b ( Taf4b ) were shown to exhibit a diminished ovarian reserve. However, potential oocyte-intrinsic functions of TAF4b have not been examined. Here we use a combination of gene expression profiling and chromatin mapping to characterize the TAF4b gene regulatory network in mouse oocytes. We find that Taf4b -deficient oocytes display inappropriate expression of meiotic, chromatin, and X-linked genes, and unexpectedly we found a connection with Turner Syndrome pathways. Using Cleavage Under Targets and Release Using Nuclease (CUT&RUN), we observed TAF4b enrichment at genes involved in meiosis and DNA repair, some of which are differentially expressed in Taf4b -deficient oocytes. Interestingly, TAF4b target genes were enriched for Sp/KLF family motifs rather than TATA-box, suggesting an alternate mode of promoter interaction. Together, our data connects several gene regulatory nodes that contribute to the ovarian reserve.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":219083,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9509,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":817217,"name":"Soňa Relovská","orcid":"0000-0001-9363-1407","position":1,"is_corresponding":false},{"id":817218,"name":"Kimberly M. Abt","orcid":"0000-0002-3066-3222","position":2,"is_corresponding":false},{"id":441034,"name":"Kimberly A. Seymour","orcid":null,"position":3,"is_corresponding":false},{"id":674132,"name":"Tong Wu","orcid":"0000-0001-6613-7024","position":4,"is_corresponding":false},{"id":817219,"name":"Haskan Kaya","orcid":"0000-0002-2399-5413","position":5,"is_corresponding":false},{"id":686333,"name":"James M. A. Turner","orcid":"0000-0003-1722-7677","position":6,"is_corresponding":false},{"id":68983,"name":"Thomas G. Fazzio","orcid":"0000-0002-0353-7466","position":7,"is_corresponding":false},{"id":440247,"name":"Richard N. Freiman","orcid":"0000-0002-7269-3630","position":8,"is_corresponding":false},{"id":490412,"name":"Megan A. Gura","orcid":"0000-0001-9635-4046","position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:53:33.915158Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}