{"doi":"10.1101/2021.06.29.450363","title":"Nascent alt-protein chemoproteomics reveals a repressor of ribosome biogenesis","abstract":"Abstract Many unannotated microproteins and alternative proteins (alt-proteins) have recently been found to be co-encoded with canonical proteins, but few of their functions are known. Motivated by the hypothesis that alt-proteins undergoing active or stress-induced synthesis could play important cellular roles, here, we developed a chemoproteomic pipeline to identify nascent alt-proteins in human cells. We identified 22 actively translated unannotated alt-proteins, one of which is upregulated after DNA damage stress. We further defined MINAS-60 ( MI croprotein that N egatively regulates AS sembly of the pre- 60 S ribosomal subunit), a nucleolar localized alt-protein co-encoded with human RBM10.Depletion of MINAS-60 increases the amount of the mature 60S ribosomal subunit, consequently upregulating global protein synthesis and cell proliferation by repressing late-stage pre-60S assembly and export of the 60S ribosome subunit to the cytoplasm. Together, these results implicate MINAS-60 as a repressor of ribosome biogenesis, and demonstrate that chemoproteomics can enable generation of functional hypotheses for uncharacterized alt-proteins.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":225404,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9542,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":310477,"name":"Alexandra Khitun","orcid":"0000-0003-4137-0035","position":1,"is_corresponding":false},{"id":697899,"name":"Cecelia M. Harold","orcid":"0000-0002-6855-8380","position":2,"is_corresponding":false},{"id":724069,"name":"Carson J. Bryant","orcid":"0000-0002-7475-875X","position":3,"is_corresponding":false},{"id":828229,"name":"Shu-Jian Zheng","orcid":null,"position":4,"is_corresponding":false},{"id":409909,"name":"Susan J. Baserga","orcid":"0000-0002-7731-8629","position":5,"is_corresponding":false},{"id":17874,"name":"Sarah A. Slavoff","orcid":"0000-0002-4443-2070","position":6,"is_corresponding":false},{"id":296654,"name":"Xiongwen Cao","orcid":null,"position":0,"is_corresponding":true}],"reference_count":65,"raw_metadata":null,"created_at":"2026-07-18T23:54:26.581453Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}