{"doi":"10.1101/2021.06.14.448361","title":"Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei","abstract":"Kinetoplastids, including Trypanosoma brucei, control gene expression primarily at the posttranscriptional level. Nuclear mRNA export is an important, but understudied, step in this process. The general heterodimeric export factors, Mex67/Mtr2, function in the export of mRNAs and tRNAs in T. brucei, but RNA binding proteins (RBPs) that regulate export processes by controlling the dynamics of Mex67/Mtr2 ribonucleoprotein formation or transport have not been identified. Here, we report that DRBD18, an essential and abundant T. brucei RBP, associates with Mex67/Mtr2 in vivo, likely through its direct interaction with Mtr2. DRBD18 downregulation results in partial accumulation of poly(A)+ mRNA in the nucleus, but has no effect on localization of intron-containing or mature tRNAs. Comprehensive analysis of transcriptomes from whole cell and cytosol in DRBD18 knockdown parasites demonstrates that depletion of DRBD18 leads to impairment of nuclear export of a subset of mRNAs. CLIP experiments reveal association of DRBD18 with several of these mRNAs. Moreover, DRBD18 knockdown leads to a partial accumulation of the Mex67/Mtr2 export receptors in the nucleus. Taken together, the current study supports a model in which DRBD18 regulates the selective nuclear export of mRNAs by promoting the mobilization of export competent mRNPs to the cytosol through the nuclear pore complex.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":218934,"datarank":0.1701479806045433,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.00535613730432685,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.00535613730432685,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9502,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":817077,"name":"Jan Naseer Kaur","orcid":"0000-0002-4808-3904","position":1,"is_corresponding":false},{"id":389652,"name":"Daniel McSkimming","orcid":null,"position":2,"is_corresponding":false},{"id":665949,"name":"Eva Hegedűsová","orcid":"0000-0002-6266-1811","position":3,"is_corresponding":false},{"id":757499,"name":"Ashutosh Prakash Dubey","orcid":"0000-0002-6817-3202","position":4,"is_corresponding":false},{"id":817078,"name":"Martı́n Ciganda","orcid":"0000-0003-3229-3365","position":5,"is_corresponding":false},{"id":665950,"name":"Zdeněk Paris","orcid":"0000-0003-1019-7719","position":6,"is_corresponding":false},{"id":273324,"name":"Laurie K. Read","orcid":"0000-0003-1235-6736","position":7,"is_corresponding":false},{"id":817076,"name":"Amartya Mishra","orcid":"0000-0003-1071-4851","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:53:33.915158Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}