{"doi":"10.1101/2021.06.02.446776","title":"Extremely bright, near-IR emitting spontaneously blinking fluorophores enable ratiometric multicolor nanoscopy in live cells","abstract":"Abstract New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multi-color, live-cell, single-molecule localization microscopy (SMLM), especially for experiments that demand ultra-high-resolution live imaging. Here we report the design, synthesis, and biological evaluation of Yale 676sb , a photostable, near-IR emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale 676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":218893,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9542,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":582225,"name":"Kevin Hu","orcid":"0000-0002-4410-574X","position":1,"is_corresponding":false},{"id":653734,"name":"Shuai Zheng","orcid":"0000-0001-9085-4410","position":2,"is_corresponding":false},{"id":259883,"name":"P. Kidd","orcid":"0000-0003-0308-5065","position":3,"is_corresponding":false},{"id":653735,"name":"Neville Dadina","orcid":"0000-0002-7118-4666","position":4,"is_corresponding":false},{"id":472066,"name":"Ling Chu","orcid":"0000-0002-4418-6850","position":5,"is_corresponding":false},{"id":299631,"name":"Derek Toomre","orcid":"0000-0002-6827-8127","position":6,"is_corresponding":false},{"id":244374,"name":"Joerg Bewersdorf","orcid":"0000-0002-4085-7020","position":7,"is_corresponding":false},{"id":237245,"name":"Alanna Schepartz","orcid":"0000-0003-2127-3932","position":8,"is_corresponding":false},{"id":472603,"name":"Jonathan Tyson","orcid":null,"position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-18T23:53:33.915158Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}