{"doi":"10.1101/2021.05.26.445893","title":"Female mice exhibit a more sensitive automated squint response to pain induced by CGRP and amylin","abstract":"ABSTRACT We developed an automated squint assay using both black C57BL/6J and white CD1 mice that measured the interpalpebral fissure area between the upper and lower eyelids as an objective quantification of pain. In C57BL/6J mice, we observed a squint response to increasing doses of a migraine trigger, the neuropeptide CGRP, including a significant response in female mice at a dose below detection by the manual grimace scale. Using the automated software, both C57BL/6J and CD1 mice lacked a detectable photic blink response. The CGRP-related peptide amylin induced squinting behavior in female mice, but not males. These data demonstrate that an automated squint assay can be used as an objective, real-time continuous-scale measure of pain that provides higher precision and real-time analysis compared to manual grimace assessments.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":218820,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.957,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":563150,"name":"Levi P. Sowers","orcid":"0000-0002-5375-9825","position":1,"is_corresponding":false},{"id":816948,"name":"Abigail Davison","orcid":"0000-0001-6695-8121","position":2,"is_corresponding":false},{"id":816949,"name":"Aaron M. Fairbanks","orcid":"0000-0002-2348-4517","position":3,"is_corresponding":false},{"id":658757,"name":"Anne‐Sophie Wattiez","orcid":"0000-0002-4392-2211","position":4,"is_corresponding":false},{"id":816950,"name":"Pieter Poolman","orcid":"0000-0001-5470-532X","position":5,"is_corresponding":false},{"id":638804,"name":"Randy H. Kardon","orcid":"0000-0002-3173-7123","position":6,"is_corresponding":false},{"id":273685,"name":"Andrew F. Russo","orcid":"0000-0002-8156-5649","position":7,"is_corresponding":false},{"id":635186,"name":"Brandon J. Rea","orcid":"0000-0002-0412-5412","position":0,"is_corresponding":true}],"reference_count":24,"raw_metadata":null,"created_at":"2026-07-18T23:53:33.915158Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}