{"doi":"10.1101/2021.05.12.443716","title":"Injectable Progranulin-derivative Atsttrin loaded Protein Engineered Gels for Post Traumatic Osteoarthritis","abstract":"Abstract Protein-based biomaterials offer several advantages over synthetic materials, owing to their unique stimuli-responsive properties, biocompatibility and modular nature. We have successfully developed protein block polymers that consist of elastin like polypeptide (E) and the coiled-coil domain of cartilage oligomeric matrix protein (C). Here, we demonstrate that E 5 C, a construct consisting of five repeats of E and a single domain of C, is capable of forming a porous networked gel at physiological temperature, making it an excellent candidate for injectable biomaterials. Combination of E 5 C with Atsttrin, a chondroprotective engineered derivative of anti-inflammatory growth factor progranulin (PGRN), provides a unique biochemical and biomechanical environment to protect against post-traumatic osteoarthritis (PTOA) onset and progression. E 5 C gel was demonstrated to provide prolonged release of Atsttrin and inhibit chondrocyte catabolism while facilitating anabolic signaling in vitro . We also provide in vivo evidence that prophylactic and therapeutic application of Atsttrin-loaded E 5 C hydrogels protected against PTOA onset and progression in a rabbit anterior cruciate ligament transection model. Collectively, we have developed a unique protein-based gel capable of minimally invasive, sustained delivery of prospective therapeutics, particularly the PGRN-derivative Atsttrin, for prevention of OA onset and progression.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":224262,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9488,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":417960,"name":"Aubryanna Hettinghouse","orcid":"0000-0002-7596-6315","position":1,"is_corresponding":false},{"id":826265,"name":"Michael Meleties","orcid":"0000-0003-1621-9835","position":2,"is_corresponding":false},{"id":826266,"name":"Changhong Chen","orcid":"0000-0001-8588-5853","position":3,"is_corresponding":false},{"id":776956,"name":"Min Cui","orcid":"0000-0002-6387-2426","position":4,"is_corresponding":false},{"id":640892,"name":"Guodong Sun","orcid":"0000-0001-5336-368X","position":5,"is_corresponding":false},{"id":826267,"name":"Sadaf Hasan","orcid":"0000-0002-8270-2705","position":6,"is_corresponding":false},{"id":713272,"name":"Rajiv Menon","orcid":"0000-0001-6160-886X","position":7,"is_corresponding":false},{"id":769324,"name":"Bonnie Lin","orcid":"0000-0003-1959-3531","position":8,"is_corresponding":false},{"id":371096,"name":"Ravinder R. Regatte","orcid":"0000-0002-4607-7682","position":9,"is_corresponding":false},{"id":310622,"name":"Liu C","orcid":"0000-0002-7181-8032","position":10,"is_corresponding":false},{"id":826268,"name":"Jin Kim Montclare","orcid":"0000-0001-6857-3591","position":11,"is_corresponding":false},{"id":826264,"name":"Priya Katyal","orcid":"0000-0003-3066-3537","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-18T23:54:18.470602Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}