{"doi":"10.1101/2021.05.08.443267","title":"An intranasal vaccine durably protects against SARS-CoV-2 variants in mice","abstract":"ABSTRACT SARS-CoV-2 variants that attenuate antibody neutralization could jeopardize vaccine efficacy and the end of the COVID-19 pandemic. We recently reported the protective activity of a single-dose intranasally-administered spike protein-based chimpanzee adenovirus-vectored vaccine (ChAd-SARS-CoV-2-S) in animals, which has advanced to human trials. Here, we assessed its durability, dose-response, and cross-protective activity in mice. A single intranasal dose of ChAd-SARS-CoV-2-S induced durably high neutralizing and Fc effector antibody responses in serum and S-specific IgG and IgA secreting long-lived plasma cells in the bone marrow. Protection against a historical SARS-CoV-2 strain was observed across a 100-fold vaccine dose range and over a 200-day period. At 6 weeks or 9 months after vaccination, serum antibodies neutralized SARS-CoV-2 strains with B.1.351 and B.1.1.28 spike proteins and conferred almost complete protection in the upper and lower respiratory tracts after challenge. Thus, in mice, intranasal immunization with ChAd-SARS-CoV-2-S provides durable protection against historical and emerging SARS-CoV-2 strains.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":213852,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9532,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":106516,"name":"Swathi Shrihari","orcid":"0000-0002-8188-6936","position":1,"is_corresponding":false},{"id":321856,"name":"Matthew J. Gorman","orcid":null,"position":2,"is_corresponding":false},{"id":408059,"name":"Baoling Ying","orcid":null,"position":3,"is_corresponding":false},{"id":628510,"name":"Dansu Yuan","orcid":"0000-0003-4815-7237","position":4,"is_corresponding":false},{"id":564287,"name":"Saravanan Raju","orcid":"0000-0003-3216-5574","position":5,"is_corresponding":false},{"id":106500,"name":"Rita E. Chen","orcid":"0000-0002-5100-7750","position":6,"is_corresponding":false},{"id":523835,"name":"Igor P. Dmitriev","orcid":"0000-0001-9206-1975","position":7,"is_corresponding":false},{"id":524668,"name":"Elena A. Kashentseva","orcid":null,"position":8,"is_corresponding":false},{"id":246334,"name":"Lucas J. Adams","orcid":"0000-0002-1724-8120","position":9,"is_corresponding":false},{"id":38215,"name":"Pei‐Yong Shi","orcid":"0000-0001-5553-1616","position":10,"is_corresponding":false},{"id":225534,"name":"Daved H. Fremont","orcid":"0000-0002-8544-2689","position":11,"is_corresponding":false},{"id":314553,"name":"David T. Curiel","orcid":"0000-0003-3802-6014","position":12,"is_corresponding":false},{"id":106757,"name":"Galit Alter","orcid":"0000-0002-7680-9215","position":13,"is_corresponding":false},{"id":106529,"name":"Michael Diamond","orcid":"0000-0002-8791-3165","position":14,"is_corresponding":false},{"id":106512,"name":"Ahmed O. Hassan","orcid":"0000-0002-9638-1260","position":0,"is_corresponding":true}],"reference_count":72,"raw_metadata":null,"created_at":"2026-07-18T23:52:41.672172Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}