{"doi":"10.1101/2021.05.06.441257","title":"Vps501, a novel vacuolar SNX-BAR protein cooperates with the SEA complex to induce autophagy","abstract":"Abstract The sorting nexins (SNX), constitute a diverse family of molecules that play varied roles in membrane trafficking, cell signaling, membrane remodeling, organelle motility and autophagy. In particular, the SNX-BAR proteins, a SNX subfamily characterized by a C-terminal dimeric Bin/Amphiphysin/Rvs (BAR) lipid curvature domain and a conserved Phox-homology domain, are of great interest. In budding yeast, many SNX-BARs proteins have well-characterized endo-vacuolar trafficking roles. Phylogenetic analyses allowed us to identify an additional SNX-BAR protein, Vps501, with a novel endo-vacuolar role. We report that Vps501 uniquely localizes to the vacuolar membrane and works with the SEA complex to regulate autophagy. Furthermore, we found cells displayed a severe deficiency in starvation-induced/nonselective autophagy only when SEA complex subunits are ablated in combination with Vps501, indicating a cooperative role with the SEA complex during autophagy. Additionally, we found the SEA complex becomes destabilized in vps501 Δ sea1 Δ cells, which resulted in aberrant TORC1 hyperactivity and misregulation of autophagy induction.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":220640,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9629,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":478600,"name":"Verónica A. Segarra","orcid":"0000-0001-6914-8271","position":1,"is_corresponding":false},{"id":444785,"name":"Nitika Nitika","orcid":null,"position":2,"is_corresponding":false},{"id":820358,"name":"Aaron M. Stetcher","orcid":null,"position":3,"is_corresponding":false},{"id":444005,"name":"Andrew W. Truman","orcid":"0000-0002-6409-3723","position":4,"is_corresponding":false},{"id":510200,"name":"Adam M. Reitzel","orcid":"0000-0001-5734-7118","position":5,"is_corresponding":false},{"id":820104,"name":"J. Richard","orcid":"0000-0003-4830-6685","position":6,"is_corresponding":false},{"id":820357,"name":"Shreya Goyal","orcid":null,"position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:53:46.965811Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}