{"doi":"10.1101/2021.04.25.441362","title":"RNA-specific condensation promotes auto-regulation of polyQ RNA-binding protein Whi3","abstract":"Abstract RNA-binding proteins are frequently seen to be capable of auto-regulation by binding their own transcripts. In this work, we show that in the multinucleated fungus Ashbya gossypii , the phase-separating RNA-binding protein Whi3 binds and regulates its own transcripts in distinct condensates from its other targets. Failure of Whi3 to bind its own transcript leads to a reduction in Whi3 protein level and its inability to properly regulate its other targets, leading to defects in nuclear cycling, polarized growth, and transcription of START-regulated genes. These results present a role for auto-regulation and condensate formation triggered by an RNA-binding protein interacting with its own coding transcript. Given the propensity of RNA-binding proteins to interact with their own coding mRNAs, this may be a wide-spread mechanism of feedback that utilizes biomolecular condensates.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":223994,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9595,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":335245,"name":"Erin M. Langdon","orcid":"0000-0002-9324-6215","position":1,"is_corresponding":false},{"id":825774,"name":"Grace McLaughlin","orcid":"0000-0002-5246-7820","position":2,"is_corresponding":false},{"id":309244,"name":"Benjamin M. Stormo","orcid":"0000-0002-6861-8451","position":3,"is_corresponding":false},{"id":826112,"name":"Blair Yu","orcid":null,"position":4,"is_corresponding":false},{"id":564717,"name":"Marcus Roper","orcid":"0000-0001-8756-0112","position":5,"is_corresponding":false},{"id":5955,"name":"Amy S. Gladfelter","orcid":"0000-0002-2490-6945","position":6,"is_corresponding":false},{"id":393302,"name":"Therese M. Gerbich","orcid":"0000-0003-4232-9536","position":0,"is_corresponding":true}],"reference_count":15,"raw_metadata":null,"created_at":"2026-07-18T23:54:14.760456Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}