{"doi":"10.1101/2021.04.11.438056","title":"The H4K20 demethylase DPY-21 regulates the dynamics of condensin DC binding","abstract":"ABSTRACT Condensin is a multi-subunit SMC complex that binds to and compacts chromosomes. Here we addressed the regulation of condensin binding dynamics using C. elegans condensin DC, which represses X chromosomes in hermaphrodites for d osage c ompensation. We established fluorescence recovery after photobleaching (FRAP) using the SMC4 homolog DPY-27 and showed that a well-characterized ATPase mutation abolishes its binding. Next, we performed FRAP in the background of several chromatin modifier mutants that cause varying degrees of X-chromosome derepression. The greatest effect was in a null mutant of the H4K20me2 demethylase DPY-21, where the mobile fraction of condensin DC reduced from ∼30% to 10%. In contrast, a catalytic mutant of dpy-21 did not regulate condensin DC mobility. Hi-C data in the dpy-21 null mutant showed little change compared to wild type, uncoupling Hi-C measured long-range DNA contacts from transcriptional repression of the X chromosomes. Together, our results indicate that DPY-21 has a non-catalytic role in regulating the dynamics of condensin DC binding, which is important for transcription repression.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":223860,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9558,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":646477,"name":"Ana Karina Morao","orcid":"0000-0003-2194-8071","position":1,"is_corresponding":false},{"id":750122,"name":"Jun Kim","orcid":"0000-0001-9473-3093","position":2,"is_corresponding":false},{"id":750123,"name":"David Sebastian Jimenez","orcid":"0000-0003-0943-9063","position":3,"is_corresponding":false},{"id":750124,"name":"Nina Maryn","orcid":"0000-0002-0975-3354","position":4,"is_corresponding":false},{"id":750125,"name":"Krishna Bikkasani","orcid":"0000-0002-4000-1863","position":5,"is_corresponding":false},{"id":55212,"name":"Michael J. Carrozza","orcid":null,"position":6,"is_corresponding":false},{"id":750126,"name":"Sarah Elizabeth Albritton","orcid":"0000-0001-6093-9560","position":7,"is_corresponding":false},{"id":750127,"name":"Maxwell Kramer","orcid":"0000-0002-1643-0756","position":8,"is_corresponding":false},{"id":750128,"name":"Lena Annika Street","orcid":"0000-0002-5473-6713","position":9,"is_corresponding":false},{"id":750129,"name":"Kustrim Cerimi","orcid":"0000-0003-0775-3032","position":10,"is_corresponding":false},{"id":750130,"name":"Vic-Fabienne Schumann","orcid":"0000-0003-0227-7428","position":11,"is_corresponding":false},{"id":750131,"name":"Ella Bahry","orcid":"0000-0002-6358-2576","position":12,"is_corresponding":false},{"id":52909,"name":"Stephan Preibisch","orcid":"0000-0002-0276-494X","position":13,"is_corresponding":false},{"id":65112,"name":"Andrew Woehler","orcid":"0000-0001-5157-9313","position":14,"is_corresponding":false},{"id":23423,"name":"Sevinç Ercan","orcid":"0000-0001-7297-1648","position":15,"is_corresponding":false},{"id":750121,"name":"Laura Breimann","orcid":"0000-0001-7702-5749","position":0,"is_corresponding":true}],"reference_count":91,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:54:14.760456Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}