{"doi":"10.1101/2021.03.23.21254201","title":"Toward Community Surveillance: Detecting Intact SARS-CoV-2 Using Exogeneous Oligonucleotide Labels","abstract":"Abstract The persistence of the COVID-19 pandemic demands a dramatic increase in testing efficiency. Testing pooled samples for SARS-CoV-2 could meet this need; however, the sensitivity of RT-qPCR, the gold standard, significantly decreases with an increasing number of samples pooled. Here, we introduce DIVER, a method that quantifies intact virus and is robust to sample dilution. DIVER first tags viral particles with exogeneous oligonucleotides, then captures the tagged particles on ACE2-functionalized beads, and finally quantifies the oligonucleotide tags using qPCR. Using spike-presenting liposomes and Spike-pseudotyped lentivirus as SARS-CoV-2 models, we show that DIVER can detect 1×10 5 liposomes and 100 pfu lentivirus and can successfully identify positive samples in pooling experiments. Overall, DIVER is well-positioned for efficient sample pooling and expanded community surveillance.","journal":"medRxiv","year":2021,"id":220486,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9585,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":459134,"name":"Molly Kozminsky","orcid":"0000-0001-7040-1641","position":1,"is_corresponding":false},{"id":819994,"name":"Jennifer C. Hall","orcid":"0000-0002-3524-6647","position":2,"is_corresponding":false},{"id":819995,"name":"Valerie Vargas-Zapata","orcid":"0000-0002-5356-8703","position":3,"is_corresponding":false},{"id":513523,"name":"Kristina Geiger","orcid":"0000-0001-6738-7439","position":4,"is_corresponding":false},{"id":561536,"name":"Laurent Coscoy","orcid":"0000-0002-7337-2345","position":5,"is_corresponding":false},{"id":459137,"name":"Lydia L. Sohn","orcid":"0000-0003-2195-2521","position":6,"is_corresponding":false},{"id":784526,"name":"Thomas R. Carey","orcid":"0000-0002-6752-1404","position":0,"is_corresponding":true}],"reference_count":43,"raw_metadata":null,"created_at":"2026-07-18T23:53:46.965811Z","pmid":"33791715","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}